Hello
Today i want to ask about whether there is optimal condition of centrifugation
(speed, time, and temperature) to obtain cell pellet.
I have already searched many protocols, but i only can find those mentioning about bacterial culture.
What i want to ask is about the optimal condition of centrifugation to get pellet of HUVECs. For me, i collect HUVECs to 1.5ml tubes with 1X PBS and I spin down the samples with small centrifuge for about just 30 seconds.
Then i can see the pellet at the bottom.
But i learned that there are numerous conditions of pelleting down cells
so i want to ask whether my protocol described above is ok
Thank you very much!
Thanks for all of your information.
Gratitude to Hasan Al Faruque, Aziz Nakhanov, Serena Lorini.
But what i wanted to ask was about whether pellet formed by my protocol
described above is HUVEC cells rather than various conditions of centrifugation.
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