I'm having a hard time finding a reference for my specific conditions, any help would be appreciated:
I have dsDNA bound to streptavidin-coated magnetic beads via a biotin interaction. The dsDNA is covalently attached to the biotin via a psoralen crosslink. The conditions I want to use are (10 mM Tris (pH 7.6), 0.4 mM EDTA, 100 mM
KOH, 90 C for 30 minutes) to reverse the psoralen crosslink. Under these conditions, only one of the DNA strands will be released from the psoralen, leaving the complementary strand still covalently attached to the psoralen-biotin linker.
My question is: Will these conditions also inactivate the biotin-streptavidin interaction allowing for release of the complementary strand (at the same time) and allow for recovery of uncrosslinked-dsDNA? Or will I only recover ssDNA, with the complementary strand still attached to the magnetic beads?
Note: I am using Dynabeads MyOne C1, streptavidin coated magnetic beads