10 February 2017 4 3K Report

I ran samples (looking for c.bovis in swabs and tumors) using the same primes/probes, Taqman Master Mix, DEPC H2O that was used last week on samples that worked fine. These new samples that should had been negative tested positive for c. bovis. I replaced all reagents and reran the samples. Still positive. Some samples are negative and the NTC is negative so I do not thing contamination is the cause. I can not think of any other cause for the false positives. Can anyone offer any assistance? Thank You.

I have also included an image of the results for better visual. 

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