24 February 2017 14 3K Report

I have transformed my cloned plasmid DNA into bacteria and cultured bacteria. Then, I extracted DNA from bacteria and run in 0.5% agarose gel. But I am not seeing any DNA band except ladder.

(In nanodrop, the conc. was 86ng/microliter, but 260/280 ratio was 1.62)

To check the gel, I had run another DNA besides this DNA. But I found the band of another DNA.

What could be the problem?

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