I have transformed my cloned plasmid DNA into bacteria and cultured bacteria. Then, I extracted DNA from bacteria and run in 0.5% agarose gel. But I am not seeing any DNA band except ladder.
(In nanodrop, the conc. was 86ng/microliter, but 260/280 ratio was 1.62)
To check the gel, I had run another DNA besides this DNA. But I found the band of another DNA.
What could be the problem?