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20 October 2023 0 5K Report

Identification, characterization and selection of autochthonous lactic acid bacteria as probiotic for feedlot cattle

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WST-1 test for 3D cell culture?

I want to perform WST-1 test with alginate based 3D-colorectal cancer cells. I wonder about before performing WST-1 test, ıs it necessary to remove alginate spheres from the cells (to carry it...

29 June 2024 9,667 1 View

Is there anyone using EvolveAGene4?

Hi, I am trying to run EvolveAGene4 program, I can open the program, creating path, commands are working, however; I am getting this error message: "The sequence must be a coding sequence whose...

11 May 2024 7,709 0 View

LPS 2 µg/µL?

How can I dilute my LPS to 2 µg/µL stock solution?

19 March 2024 1,346 3 View

How to make LPS 10 mg/kg ?

How can I prepare LPS solution to inject 20g mice with 10 mg/kg LPS ?

12 March 2024 7,163 1 View

Request for help with methodology ''senescence in adipocytes'' ?

We want to study senescence in adipocytes. However, we applied the method at adipose cells in our laboratory was as follows; ''Preadipocyte cell line 3T3-L1 (American Type Culture Collection,...

03 January 2024 2,653 1 View

I want a full pdf?

Probiotics in cattle - J. T. Huber

16 July 2023 1,041 4 View

How can ı work on polymer cif file, ı want to prepare energy framework calculation with CrystalExplorer21.5?

I use crsytal explorer, my cif file open than ı choose all atoms but the energy calculation button is not active, what can I do?

28 March 2023 8,471 1 View

I'm getting a tonto error in Crystal Explorer, how can I solveit?

T O N T O A free object-based system for computational chemistry Version: 3.2 rev. 4048 Platform: GNU-gfortran-on-WINDOWS Build-date: Tue, Jun 11, 2013 8:18:30 AM For conditions...

27 February 2023 8,318 0 View

How can I solve "admissibility check" problem with LISREL?

I am using LISREL 8.50 and during SEM anaysis program gives me an error like "The solution was found non-admissible after 50 iterations. The following solution is preliminary and is provided only...

21 November 2021 2,805 4 View

Caki-I cell line and Caki-II cell line?

I have a problem with clear renal cell carcinoma cell lines Caki-I and Caki-II. I can not proliferate them. Are there anybody who studied these cell lines before? Can somebody give some advice....

08 March 2021 1,943 2 View

How does the human microbiome influence health and disease, including implications for personalized medicine and microbiome-targeted therapies?

Probiotics related.

22 July 2024 1,707 2 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

How to ask to correct the Incorrect Claim regarding the writer of Article that I was involved in?

Dear sir/Madam, I am Parinaz Parsa the first writer of article "Optimisation of probiotic yoghurt production enriched with phytosterols" and understand that one person is mentioned as writer which...

14 July 2024 9,579 2 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

Can all postbiotics survive the gastric journey or is it again strain-specific or will depend the type of postbiotic?

General understanding is that postbiotic particularly those that are heat-treated are stable to heat and their activity remains stable during gastric journey. Wondering if this is true for all...

25 June 2024 3,133 3 View

Did you ASK AI (https://talkai.info/tr/chat/) the same question?

What food do intestinal bacteria like most?ChatGPTIntestinal bacteria love fibrous foods that are prebiotic. These foods include vegetables (such as broccoli, cabbage, and brussels sprouts),...

22 June 2024 4,567 0 View

Can increasing bacterial concentration in a formulation compensate for inhibition by lactobacillus strain,and if so ,is it right to do so?

I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...

01 June 2024 338 2 View

What is the function of CaCl2 in the lipolytic assay? what percentage concentration of CaCl2 should be added in the lipolytic assay?

Hello everyone. I need some information about the use of CaCl2 in lipolytic assays. I often see the addition of CaCl2 in lipolytic activity assays, especially in probiotic screening, but there is...

08 May 2024 7,480 2 View