15 August 2024 1 5K Report

if available on addgene

More Tanuj Handa's questions See All
Could you suggest some article on classification of fixing scheme of workpiece for milling operation?

The scheme of fixing the workpiece during milling operation.

13 January 2023 6,437 3 View

Neutral state of dynamical system?

Hello all, Is it possible to have multiple (more than 3) neutral-state (center) in a dynamical system? If yes, please provide some references. Thank you.

09 October 2022 4,556 1 View

Please assist in solving Molecular dynamic simulation of protein-ligand covalently bound complex using GROMACS?

I have been working on the design of covalent inhibitors, which are covalently bound to the cysteine side chain. it would be really helpful if somebody would guide how to modify the processed...

06 March 2022 6,794 3 View

I want to know some research topics in "Design and technological support of machine-building industries" Automobile Technology ?

Respected, Kindly suggest some topics on the above-mentioned course, I am also interested in the design and simulation, of Automobile parts and assembly.

05 November 2021 7,442 2 View

Changing axis of rotation in UDF?

Sir, I am moving my object as per the SHM like (omega = a sin(wt)). what change do I need to make in UDF to change the axis of rotation from origin to some other axis say at (x,y) = (1,1)? For UDF...

10 December 2020 213 0 View

Gravity variation in UDF fluent?

Hello members, If I have a body that is oscillating with some time period, due to which I need to change the gravity component as per the motion like "g_x = g*cos (theta) or g_y = g*sin (theta)"...

09 February 2020 4,623 1 View

How to find the residual stresses in carbon fibre composites. Will XRD work for the same?

Respected all, I want to know about the correct method to find out the residual stresses in the carbon fibre reinforced composites. The literature says that XRD can get residual stresses. However,...

30 January 2020 689 13 View

How to create moving reference monitor point?

I am working on 2-D domain which is rotating as per the UDF I created for SHM motion. I created a line to measure say average temperature along the line. But as the domain rotate line remains...

23 January 2020 5,779 0 View

Query related to Zone motion?

If there is no interface in the domain, is it advisable to use mesh motion along with frame motion? Because if I am using only frame motion then there occurs no motion in animation. While using a...

20 January 2020 1,359 0 View

Difference between mass flow rate calculation?

In my 3-D simulation of natural convection flow, I calculated mass flow rate using Fluent and CFD-Post. There is a significant difference between the value of both methods, though they belong to...

02 January 2020 5,098 3 View

Similar questions and discussions
How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

How to solve g_mmpbsa error?

Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out

07 August 2024 6,066 0 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

JCPDS 65-7246 file please?

Dear Researchers Kindly share JCPDS 65-7246 file Thanks in advance

04 August 2024 5,613 1 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close