Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures. | Contact experts in Membranes to get answers
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Questions related to Membranes
Hello I work in sw620, sw480 and HT 29 cell lines and I did a migration assay for 29 H using Dapi . the problem is that i didn't get enough fluoresence I put a 200000 cell in 200µl free medium...
01 March 2021 2,788 2 View
I want to measure water flux across the membrane. This water flux is described here: https://pubs.acs.org/doi/10.1021/acs.jpcb.6b01870 "Water flux across the membrane Based on the number of...
12 February 2021 6,976 0 View
I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption
09 February 2021 7,922 3 View
Greetings, I have created a number of 100ns ligand and enzyme simulations in which the simulation begins with my ligand within the binding pocket, then over the course of anywhere from 5-20ns the...
30 January 2021 3,789 2 View
Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...
28 January 2021 8,077 1 View
I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...
28 January 2021 1,232 3 View
I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...
28 January 2021 5,313 2 View
I isolated a few bacteria from fouled RO membrane and I want to check whether any of them produces AHL signals. I did cross streak assay with cvo26 but none of the bacteria showed a positive...
21 January 2021 2,209 0 View
Hi! I would like to study 5-HT 1B (Serotonin 1B) receptors and conduct a Gi CAMP assay on it. As you know, this is a functional assay that requires whole cells and not membrane preparations. Do...
20 January 2021 3,715 2 View
I am trying to collect at least 10 uL of plasma from around 75 uL of whole blood without using a centrifuge. I also need to be able to collect the plasma somehow for separate analysis. How can I...
19 January 2021 4,611 1 View
Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...
15 January 2021 9,353 4 View
Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...
12 January 2021 6,147 4 View
I´m working with flower cells of a plant that only flourish once a year, can you recommend me a fluorescent dye that can be used in fixed cells that allows me to visualize de membrane of the...
08 January 2021 4,971 2 View
Recycled vertical flow constructed wetland (RVFCW), Membrane bio Reactor or simply constructed wetland.in small communities and household, please mention with description
31 December 2020 6,359 4 View
I modified PES membranes with nanoparticles (nanosilver, graphene, halloysite). They were used in UF for WWTP effluent treatment. After filtration, all membranes exhibited more negative zeta...
27 December 2020 5,814 3 View
I had been having beautiful blot transfers, but in the last few days I have ran into issues where there is high background staining and poor band resolution. The background staining is very...
24 December 2020 823 3 View
Hey community, in the next months I will measure the permeability and selectivity towards different gases of different tubular membranes in the lab scale. I have to compare the results to my own...
18 December 2020 5,025 3 View
Hi everyone I'm currently working with a membrane transporter protein to which I need to simulate molecular dynamics (MD). For this, I have inserted my protein in a membrane with CHARMM-GUI and...
17 December 2020 593 1 View
Dear Collegues, I am using a relevant number of centrifugal filtration tubes made of polyethersulfone (PES) with 2 different MWCO, 5 and 50kDa for separation of protein in milk. They are quite...
16 December 2020 8,054 3 View
In the Desalination Pretreatment Facility, what is the mechanism and chemical reagents used in Dissolved Air Flotation as pretreatment of water? #DAF #RO #Water #Desalination #pretreatment #membrane
15 December 2020 1,438 6 View
Hi, I was trying to obtain coumarin6 release profile from polymeric NPs. However, I observed significant loss to the dialysis membrane/centrifuge tubes. Any suggestion on type of material or tube...
10 December 2020 7,055 1 View
Hi all, I'm working on RAB10, which is a small RabGTPase and is geranylgeranylated at his c-terminal cysteine residues. This is important for membrane insertion. I'm wondering if this...
05 December 2020 4,088 1 View
I have been measuring the conductivity of the biopolymer membrane by using the 2-electrodes connection and sometimes the plot intersects the a-axis and other times doesn't. The membrane was...
04 December 2020 8,647 1 View
I blocked with skimmed milk, did the 1ry Abs incubation overnight at 4C. I have used two Abs (two membrane) for detection of integrin b1 using western blotting. Washed 5 times with TBST, each is...
01 December 2020 4,304 3 View