Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures. | Contact experts in Membranes to get answers
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Questions related to Membranes
Our lab is thinking of using a standard office scanner to image coomassie blue stained SDS gels and ponceaus S western blot membranes. We are thinking of using one like this one...
28 September 2021 4,165 2 View
Hallo. We are working on the simulation of membrane for the upgrading of biogas, and we read about a useful "tool" for the simulation that is called "Chembrane", which can be interfaced to Aspen...
27 September 2021 4,713 0 View
I am working on Vanadium redox flow batteries (VRFB). In other redox flow batteries there is a problem of cross contamination of redox species across membrane. I am using nafion 112 in VRFB and...
25 September 2021 2,842 1 View
Hey Guys, I would like to grow two types of cells in a single 96 plate well and allow for secreted factors to pass between a vertical permeable membrane. I want to use robotics so it would be...
21 September 2021 1,606 13 View
To deactivate an endoplasmic membrane enzyme, which is actually the last and key enzyme of a complex enzymatic reaction, I want to use the carboxyl group deletion.But I do not want to cause...
19 September 2021 6,810 16 View
Hello researchers I am working on cell culture. I have to make solution of phosphatidic acid. Anyone can guide me how to make its solution in chloroform (solubility of phosphatidic acid) and later...
12 September 2021 1,811 0 View
Hello everyone, I am trying to perform cell migration assay for OPCs, I have a question regarding coating transwell insert. Does the membrane need to be coated with laminin on both sides of the...
09 September 2021 5,158 1 View
I am now working with polysulfone composite membrane.
08 September 2021 6,785 3 View
Hi everyone, I have gone through many papers of amyloid and phospholipid membrane interaction. 1. What is monomeric and oligomeric state of amyloid doi:10.1016/S0006-291X(03)00386-3 Biophysical...
06 September 2021 6,462 2 View
I already know the commercial trackers and calnexin, calreticulin, and KDEL, but I was looking for a marker more specifical of the membrane itself, and not of its lumen, neither signal peptides.
03 September 2021 3,204 3 View
Nitro cellulose membrane(NC membrane) contains serveral nitro groups as functional group ... am trying to convert nitro group to amino group... please suggest me any method for membrane reduction...
23 August 2021 5,593 4 View
We measure the activity of beta-lactamase enzyme inside bacterial outer membrane vesicles obtained by ultrafiltration of culture supernatants. Beta-lactamase activity value is expressed as mU/mg...
23 August 2021 6,497 4 View
After performing dialysis on nitrocellulose membrane (Slide-A-Lyzer MINI device, MWCO = 20K) concentration of my protein (CmGRP) dropped basically to zero. It is most likely that protein sticks to...
15 August 2021 2,245 2 View
I already use H2SO4 as a crosslinker but when I wash it with NaOH (before wash it again and soak with H2SO4), it becomes wreck and damage.
13 August 2021 2,954 5 View
The resistance of the sharp electrode would be roughly 100MOhm. The goal is to study tracer coupling pattern of some retinal cells.
13 August 2021 1,701 1 View
I am developing a gas diffusion layer（ORR cathode） One side of the diffusion layer is inert gas (containing trace oxygen), and the other side is (1M) KOH. I hoped that there will be high...
11 August 2021 4,130 2 View
I have been trying to extract protein of the human amniotic membrane by diferent methods but no one turned out a good option. I need to run Elisa tests to detect growth factors and tried the...
07 August 2021 2,468 4 View
Good evening everybody; I am doing a research to produce a nanofiber membrane for HEPA (H14) filter production via electrospinning. I have some questions: 1. I am spinning on a nonwoven...
03 August 2021 7,315 2 View
Given that whole blood contains blood cells in addition to the components found in blood plasma or serum, I am assuming the LOD (limit of detection) in terms of concentration of a specific antigen...
01 August 2021 9,497 0 View
Hello, I am currently doing a project related to cryo preservation and I need to ensure that the mitochondria in the cells preserved using my method stays intact (i.e., the mitochondrial...
22 July 2021 7,152 7 View
Hello everyone, I hope you are well, I really need an article or some review on the effect of temperature on the hollow fibers of a membrane. Does anyone here have any answers on this effect...
16 July 2021 7,587 7 View
Hello, I am using thp-1 cells on a cyclic olefin support for confocal microscopy. After. 2% formaldehyde staining , three washings,1% bsa and I stain them with Cell membrane staining CellMask Deep...
15 July 2021 1,344 1 View
Hello scientific comunity. Does anyone know if there is a relationship between water contact angle and membrane configuration (dense, porous, and asymmetric)? Does the water contact angle suffer...
08 July 2021 6,644 5 View
Hello, I have a ~2 g frozen pellet of CHO cells overexpressing a membrane protein (stored at -80°C) which will be used for membrane vesicles preparation. I need to resuspend the pellet, split it...
05 July 2021 2,065 5 View