Hey Guys,
I would like to grow two types of cells in a single 96 plate well and allow for secreted factors to pass between a vertical permeable membrane. I want to use robotics so it would be easier to have a vertical separation as opposed to a stack or insert like the transwells. Please let me know if anyone has encountered such a piece of plasticware.
These Corning Transwell don't really work that well https://www.fishersci.se/shop/products/10452012/10452012
or Millipore millicell also are not vertically separated
https://www.fishersci.com/shop/products/emd-millipore-millicell-cell-culture-insert-plates-3/p-4376345#?keyword=
Best,
Moaraj
Hi,
I work with 96-well-membrane and it is not known to me that something like vertically membrane exist... I don't know which cells you are going to co-culture but perhaps you can separate them with gel ore something like that (seed them in gel)... Or you have a look on the homepage of ibidi.... They have slides for woundhealing and other migration assays. Or you can use there silicon insert with a separated gab in the middle for co-culture of cells: https://ibidi.com/culture-inserts/24-culture-insert-2-well.html
best regards,
Constanze
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
Hi Constanze,
I dont have the option or using gel separation since there will be pther compolenents in the media that will aggregate in gel.
I was thinking about just puttinf a nitrocellulose filter in the tranwell insert
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