How is the Limit of Detection affected in Lateral Flow Assays depending on whether you do the detection in plasma/serum or in whole blood?

Antigoni Alexandrou @Antigoni-Alexandrou

01 August 2021 0 9K Report

Given that whole blood contains blood cells in addition to the components found in blood plasma or serum, I am assuming the LOD (limit of detection) in terms of concentration of a specific antigen will be higher in whole blood samples than in plasma or serum samples? Does anyone have any experience on how much higher the lowest detectable concentration of a specific antigen may be in whole blood samples?

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