Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures. | Contact experts in Membranes to get answers
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Questions related to Membranes
I am working with 175 ul of whole blood with 90% plasma extracted. The protocol calls for 350 ul so I bring it to 350 ul with PBS. We use 350 ul lysis buffer with 35 ul proteinase... Is there any...
30 November 2020 1,173 0 View
For one of my projects, I need to perform WBs on cell lysates. The protein of interest (LRAT) is located in the endosomes and the membrane of the ER. What is the best protocol to isolate proteins...
26 November 2020 1,071 3 View
Hi, I'm performing western blots on different samples and I'm facing a some problems after eletrotrasnfer with PVDF membranes. In general, during the incubations with the primary and secondary...
19 November 2020 6,565 7 View
In my data set, I am seeing exposure to my peptide of interest leads to significant increases in action potential firing responses. But in the same cell types, I am seeing a decrease in the...
18 November 2020 5,549 4 View
Can you please provide me the reference
18 November 2020 6,266 2 View
Hello everyone, I am having troubles with the bubble point measurement to find the pore size of my self-fabricated microfiltration membrane, and I couldn't find a proper answer anywhere else. It...
17 November 2020 7,753 1 View
I am preparing a co-culture experiment in which transwell inserts will be used. According to the references, 0.4 micrometer PET membrane was selected. And different drugs were added to the upper...
16 November 2020 3,817 2 View
Designing a desalination reverse osmosis membrane system. Could someone recommend me some designing publications or a step by step calculation guide for the design? Much appreciate. Thanks :)
07 November 2020 7,696 3 View
Hi world, I'm curious about whether having to put nanofiltration on the first phase then reverse osmosis on the second phase to desalt seawater that has a roughly 35000ppm of TDS will lower down...
06 November 2020 7,685 74 View
Hi I am searching about Dynamics of cochlea membrane partial differential equation, the equation is poisson equation. The equation is ∂2z/∂x2 + ∂2z/∂y2= −P/T can I get any reference to...
05 November 2020 3,444 1 View
Hello, I wish to model a membrane (picture attached), that is mounted on a rotor at its center. Initially, there is no load except gravity, then we start rotating using the rotor. A sweep of the...
01 November 2020 9,554 3 View
I am trying to simulate deformation of spinning membrane under gravitational force on Comsol Multiphysics software. The angular velocity of membrane is changing with time. I am getting the error...
31 October 2020 8,844 2 View
I activated my membrane twice in total and hybridized it with two different antibodies. The first antibody was the specific antibody I was looking for and the second one was GAPDH. I then...
30 October 2020 5,661 6 View
I am doing voltage-clamp experiments in CA1 pyramidal cells (mEPSCs; AMPA-NMDA ratio; LTP; etc) from acute hippocampal slices in mice. I want to make sure I am only recording from excitatory...
30 October 2020 1,236 2 View
is there any work on the electrospinning of SPPSU as a membrane in the literature, thank you ?
26 October 2020 811 8 View
I am trying wrapping Ecoli BL21 cell extract into POPC liposomes but they burst in a very short time. However, when I wrap LB in the liposomes, they remain stable for a long time.(Liposomes are in...
22 October 2020 3,244 3 View
SDI indicates the fouling tendency of membrane. Is there a possibility to relate the TOC of saline water to SDI so as to measure indirectly the fouling tendency of membrane in RO desalination...
21 October 2020 8,364 4 View
I sythesis membran chitosan, membran chitosan/tripolifosfat, and membran chitosan/tannic acid/tripolifosfat. The reaction is, hydroxil (OH) of chitosan will banded with phenolik (OH) of tannic...
21 October 2020 5,542 3 View
Hello, I need to perform MD simulation for a protein embedded in a mixed membrane bilayer with certain concentrations of different lipids (sqd, dgd, and mge lipids). I didn't find tutorials for...
18 October 2020 6,566 3 View
I apologize for the analogy if you are really interested in cars. This is about protein function, but a crucial one because the extraction from membranes of a mistargeted protein is an essential...
16 October 2020 6,292 3 View
I am looking for a solvent that can dissolve PVDF but not PES (Polyether sulfone). I need to cast PVDF on PES membrane. Looks like using NMP solvent in PVDF solution after casting on PES also...
16 October 2020 9,801 4 View
Hello! I want to know how to test the electrochemical stability of the electrolyte on lithium metal. Do I only need to perform LSV? or just put electrolyte on lithium foil and see whether it is...
16 October 2020 785 4 View
Is there a way to clear stain the cytoskeleton for observation in TEM? I usually do en bloc staining(rOTO) using Drosophila. This method stains the membrane clearly but does not allow me to see...
14 October 2020 4,454 3 View
When calculating the cell potential of an electrolysis cell, does a proton exchange membrane (PEM - such as a Sustainion membrane) influence the overall cell potential, and if so, how do you...
09 October 2020 7,436 1 View