Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures. | Contact experts in Membranes to get answers
2,031 views 986 posts
Questions related to Membranes
I isolated a few bacteria from fouled RO membrane and I want to check whether any of them produces AHL signals. I did cross streak assay with cvo26 but none of the bacteria showed a positive...
21 January 2021 2,224 0 View
Hi! I would like to study 5-HT 1B (Serotonin 1B) receptors and conduct a Gi CAMP assay on it. As you know, this is a functional assay that requires whole cells and not membrane preparations. Do...
20 January 2021 3,756 2 View
I am trying to collect at least 10 uL of plasma from around 75 uL of whole blood without using a centrifuge. I also need to be able to collect the plasma somehow for separate analysis. How can I...
19 January 2021 4,623 1 View
Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...
15 January 2021 9,403 4 View
Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...
12 January 2021 6,193 4 View
I´m working with flower cells of a plant that only flourish once a year, can you recommend me a fluorescent dye that can be used in fixed cells that allows me to visualize de membrane of the...
08 January 2021 5,022 2 View
Recycled vertical flow constructed wetland (RVFCW), Membrane bio Reactor or simply constructed wetland.in small communities and household, please mention with description
31 December 2020 6,411 4 View
I modified PES membranes with nanoparticles (nanosilver, graphene, halloysite). They were used in UF for WWTP effluent treatment. After filtration, all membranes exhibited more negative zeta...
27 December 2020 5,858 3 View
I had been having beautiful blot transfers, but in the last few days I have ran into issues where there is high background staining and poor band resolution. The background staining is very...
24 December 2020 879 3 View
Hey community, in the next months I will measure the permeability and selectivity towards different gases of different tubular membranes in the lab scale. I have to compare the results to my own...
18 December 2020 5,056 3 View
Hi everyone I'm currently working with a membrane transporter protein to which I need to simulate molecular dynamics (MD). For this, I have inserted my protein in a membrane with CHARMM-GUI and...
17 December 2020 650 1 View
Dear Collegues, I am using a relevant number of centrifugal filtration tubes made of polyethersulfone (PES) with 2 different MWCO, 5 and 50kDa for separation of protein in milk. They are quite...
16 December 2020 8,098 3 View
In the Desalination Pretreatment Facility, what is the mechanism and chemical reagents used in Dissolved Air Flotation as pretreatment of water? #DAF #RO #Water #Desalination #pretreatment #membrane
15 December 2020 1,482 6 View
Hi, I was trying to obtain coumarin6 release profile from polymeric NPs. However, I observed significant loss to the dialysis membrane/centrifuge tubes. Any suggestion on type of material or tube...
10 December 2020 7,065 1 View
Hi all, I'm working on RAB10, which is a small RabGTPase and is geranylgeranylated at his c-terminal cysteine residues. This is important for membrane insertion. I'm wondering if this...
05 December 2020 4,116 1 View
I have been measuring the conductivity of the biopolymer membrane by using the 2-electrodes connection and sometimes the plot intersects the a-axis and other times doesn't. The membrane was...
04 December 2020 8,691 1 View
I blocked with skimmed milk, did the 1ry Abs incubation overnight at 4C. I have used two Abs (two membrane) for detection of integrin b1 using western blotting. Washed 5 times with TBST, each is...
01 December 2020 4,326 3 View
I am working with 175 ul of whole blood with 90% plasma extracted. The protocol calls for 350 ul so I bring it to 350 ul with PBS. We use 350 ul lysis buffer with 35 ul proteinase... Is there any...
30 November 2020 1,182 0 View
For one of my projects, I need to perform WBs on cell lysates. The protein of interest (LRAT) is located in the endosomes and the membrane of the ER. What is the best protocol to isolate proteins...
26 November 2020 1,108 3 View
Hi, I'm performing western blots on different samples and I'm facing a some problems after eletrotrasnfer with PVDF membranes. In general, during the incubations with the primary and secondary...
19 November 2020 6,605 7 View
In my data set, I am seeing exposure to my peptide of interest leads to significant increases in action potential firing responses. But in the same cell types, I am seeing a decrease in the...
18 November 2020 5,582 4 View
Can you please provide me the reference
18 November 2020 6,309 2 View
Hello everyone, I am having troubles with the bubble point measurement to find the pore size of my self-fabricated microfiltration membrane, and I couldn't find a proper answer anywhere else. It...
17 November 2020 7,804 1 View
I am preparing a co-culture experiment in which transwell inserts will be used. According to the references, 0.4 micrometer PET membrane was selected. And different drugs were added to the upper...
16 November 2020 3,855 2 View