Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures. | Contact experts in Membranes to get answers
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Questions related to Membranes
I want to measure water flux across the membrane. This water flux is described here: https://pubs.acs.org/doi/10.1021/acs.jpcb.6b01870 "Water flux across the membrane Based on the number of...
12 February 2021 7,031 0 View
I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption
09 February 2021 8,120 3 View
It would seem most efficient for aminoacyl tRNA synthetases (aaRSs) to be attached to ribosomes or anchored at endoplasmic reticulum) so that tRNAs can be immediately charged and reused. However,...
07 February 2021 2,326 0 View
Greetings, I have created a number of 100ns ligand and enzyme simulations in which the simulation begins with my ligand within the binding pocket, then over the course of anywhere from 5-20ns the...
30 January 2021 3,869 2 View
Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...
28 January 2021 8,248 1 View
I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...
28 January 2021 1,346 3 View
I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...
28 January 2021 5,518 2 View
I isolated a few bacteria from fouled RO membrane and I want to check whether any of them produces AHL signals. I did cross streak assay with cvo26 but none of the bacteria showed a positive...
21 January 2021 2,273 0 View
Hi! I would like to study 5-HT 1B (Serotonin 1B) receptors and conduct a Gi CAMP assay on it. As you know, this is a functional assay that requires whole cells and not membrane preparations. Do...
20 January 2021 3,877 2 View
I am trying to collect at least 10 uL of plasma from around 75 uL of whole blood without using a centrifuge. I also need to be able to collect the plasma somehow for separate analysis. How can I...
19 January 2021 4,649 1 View
Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...
15 January 2021 9,466 4 View
Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...
12 January 2021 6,272 4 View
I´m working with flower cells of a plant that only flourish once a year, can you recommend me a fluorescent dye that can be used in fixed cells that allows me to visualize de membrane of the...
08 January 2021 5,173 2 View
Recycled vertical flow constructed wetland (RVFCW), Membrane bio Reactor or simply constructed wetland.in small communities and household, please mention with description
31 December 2020 6,473 4 View
I modified PES membranes with nanoparticles (nanosilver, graphene, halloysite). They were used in UF for WWTP effluent treatment. After filtration, all membranes exhibited more negative zeta...
27 December 2020 5,933 3 View
I had been having beautiful blot transfers, but in the last few days I have ran into issues where there is high background staining and poor band resolution. The background staining is very...
24 December 2020 1,024 3 View
Hey community, in the next months I will measure the permeability and selectivity towards different gases of different tubular membranes in the lab scale. I have to compare the results to my own...
18 December 2020 5,115 3 View
Hi everyone I'm currently working with a membrane transporter protein to which I need to simulate molecular dynamics (MD). For this, I have inserted my protein in a membrane with CHARMM-GUI and...
17 December 2020 802 1 View
Dear Collegues, I am using a relevant number of centrifugal filtration tubes made of polyethersulfone (PES) with 2 different MWCO, 5 and 50kDa for separation of protein in milk. They are quite...
16 December 2020 8,170 3 View
In the Desalination Pretreatment Facility, what is the mechanism and chemical reagents used in Dissolved Air Flotation as pretreatment of water? #DAF #RO #Water #Desalination #pretreatment #membrane
15 December 2020 1,571 6 View
Hi, I was trying to obtain coumarin6 release profile from polymeric NPs. However, I observed significant loss to the dialysis membrane/centrifuge tubes. Any suggestion on type of material or tube...
10 December 2020 7,090 1 View
I am not able to do the cardiac specific antibody staining on my HiPSC derived cardiomyocytes. It will be very helpful, if anyone can suggest a standard protocol
10 December 2020 380 3 View
Assuming you're working on a Pump and Treat project for PFAS remediation, where water with high PFAS concentrations is purged from the source area, and cleaned ex Situ. Now my idea was to use that...
07 December 2020 1,355 3 View
Hi all, I'm working on RAB10, which is a small RabGTPase and is geranylgeranylated at his c-terminal cysteine residues. This is important for membrane insertion. I'm wondering if this...
05 December 2020 4,161 1 View