The resistance of the sharp electrode would be roughly 100MOhm. The goal is to study tracer coupling pattern of some retinal cells.
Hi,
I m using Axo-clamp 2A for intracellular injection of cortical neurons(S1/V1) with sharp microelectrodes (70Mohms) using 2% biocytin in 1MKCl (even used few times neurobiotin) in bridge mode(its similar to current-clamp mode). I think should be possible with multiclamp 700B too. You need to apply some current pulses to inject dye ( use 2nA current with the duty cycle of 400 msec on and 200 msec off for more than 10 minutes).
I have tried also tried 100Mohms microelectrodes but it was difficult for me to get good labelling with a short injection time. Please note that I m talking about intracellular single-cell injection as per my experience.
Wish you good luck.
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