Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures. | Contact experts in Membranes to get answers
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Questions related to Membranes
Dear researchers, I have a Polytetrafluoroethylene (PTFE) powder that the powders stuck together and created large particles (agglomeration). I have been able to reduce the size of the polymer...
24 December 2022 6,031 3 View
There are many processes. But overall which one is best ?
20 December 2022 5,393 5 View
Hi! I am trying to measure fluorescence intensity (calcein self-quenching) as a way to measure changes in liposome volume as a response to hyperosmotic shock. My liposomes are made from PG and...
19 December 2022 9,794 1 View
I've a protein and want to insert in lipid bilayer membrane using a set of VMD commands 1- set membrane [atomselect top all] 2- set BSAmol [mol new BSA.psf] 3- mol addfile BSA.pdb 4- set BSA...
09 December 2022 3,458 0 View
Hi, I am modelling an alkaline water electrolyzer by using multiphase flow and species transport physics. I am confused between gas and bubbles terms. From multiphase flow I can simulate the gas...
07 December 2022 4,995 2 View
I am studying polysulfone-based composite membranes using various additives.
21 November 2022 4,938 1 View
I have already tried poly L lysine coating and gelatin coating, both of them did not seem to work as well.
02 November 2022 2,242 1 View
We know charmm-gui polymer builder mostly builds neutral polymers. But what if someone wants a positively or negatively charged polymer, how can we introduce such atoms ? I know the H++ server...
26 October 2022 788 3 View
Dear researchers, We know that spiral wound, hollow fiber, and plate and frame configurations are three membrane module types. However, no particular text is about the fabrication of plate and...
21 October 2022 3,399 0 View
I am curious about PSF membrane thickness range since I want to have a membrane that can withstand high pressures. Thank you in advance for your answers!
12 October 2022 1,029 4 View
Dear researchers, I am preparing a VMD setup on a lab scale. As you know, VMD needs a vacuum pump. However, there are many types of the vacuum pumps such as: rotary vane vacuum pumps. diaphragm...
11 October 2022 441 1 View
As in reverse flow cyclone separators, flow reverses its direction at the bottom of cone. I want to know how this flow reversal takes place.
02 October 2022 7,753 3 View
hi i have a commercial pcr master mix and last month i had a sharp band and now when i used it for another sample i dont have any band? im really confused anyone can help me and tell me whats the...
11 September 2022 1,027 0 View
Protein extraction
10 September 2022 9,396 3 View
We are purifying a copper protein that should have 4 moles of copper per mole of protein, but we don't know if the protein is fully loaded with copper. We don't have atomic absorption spectroscopy...
09 June 2022 7,401 4 View
Hi, Does anyone here know of a tool or technique to measure the thickness of MDF across the entire board (4'x8') non-destructively? I currently cut up the board into 40 pieces and using a...
31 May 2022 2,534 0 View
Hi C. elegans community, I want to know the subcellular localization (at protein levels using western blotting) of two of my target proteins. For that, I am going to use Histone H4 variant as a...
18 March 2022 8,805 0 View
I have an expression plasmid which is expressed in cytosol. I want to modify that and express it in lumen of endoplasmic reticulum. Will a c terminal KDEL sequence be enough to retain it in ER or...
17 January 2022 5,105 1 View
suggest suitable software in which i can do simulation analysis using different techniques like Sol gel method or solution combustion synthesis
30 December 2021 3,292 1 View
A similar ADC HKT288 that used a DM4 payload with anti-CDH6 monoclonal was discontinued over inflammatory and neurological adverse events. Would the adverse events be tied to targeting CDH6 or to...
13 December 2021 9,359 0 View
Hi everyone, I performed spheroid sprouting assay. I stained HUVECs with Celltracker green for 30 min in ECGM basal, then generated spheroids using hanging drop method. The next day, spheroids...
10 December 2021 5,152 0 View
I am working with yeast cells in shake flasks. When I transfer cells from primary medium to secondary medium, I usually calculate the required volume that needs to be transferred on the basis of...
24 November 2021 9,293 3 View
SDI meter possesses both flow meter and pressure gauge. We just have to add our membrane in the membrane holder. My concern is that can we use this meter for membrane filtration study (to measure...
21 November 2021 2,009 4 View
I am trying to modify the Liposomal drug surface, when I tried using DSPE-PEG-Mal 2000 the binding results onto LipoDox the SDS-PAGE and Western Blot results show that very low signal, which means...
15 November 2021 2,076 0 View