Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures. | Contact experts in Membranes to get answers
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Questions related to Membranes
Nitro cellulose membrane(NC membrane) contains serveral nitro groups as functional group ... am trying to convert nitro group to amino group... please suggest me any method for membrane reduction...
23 August 2021 5,583 4 View
We measure the activity of beta-lactamase enzyme inside bacterial outer membrane vesicles obtained by ultrafiltration of culture supernatants. Beta-lactamase activity value is expressed as mU/mg...
23 August 2021 6,490 4 View
After performing dialysis on nitrocellulose membrane (Slide-A-Lyzer MINI device, MWCO = 20K) concentration of my protein (CmGRP) dropped basically to zero. It is most likely that protein sticks to...
15 August 2021 2,234 2 View
I already use H2SO4 as a crosslinker but when I wash it with NaOH (before wash it again and soak with H2SO4), it becomes wreck and damage.
13 August 2021 2,946 5 View
I am developing a gas diffusion layer(ORR cathode) One side of the diffusion layer is inert gas (containing trace oxygen), and the other side is (1M) KOH. I hoped that there will be high...
11 August 2021 4,119 2 View
I have been trying to extract protein of the human amniotic membrane by diferent methods but no one turned out a good option. I need to run Elisa tests to detect growth factors and tried the...
07 August 2021 2,458 4 View
Good evening everybody; I am doing a research to produce a nanofiber membrane for HEPA (H14) filter production via electrospinning. I have some questions: 1. I am spinning on a nonwoven...
03 August 2021 7,307 2 View
Hello, I am currently doing a project related to cryo preservation and I need to ensure that the mitochondria in the cells preserved using my method stays intact (i.e., the mitochondrial...
22 July 2021 7,136 7 View
Hello everyone, I hope you are well, I really need an article or some review on the effect of temperature on the hollow fibers of a membrane. Does anyone here have any answers on this effect...
16 July 2021 7,575 7 View
Hello, I am using thp-1 cells on a cyclic olefin support for confocal microscopy. After. 2% formaldehyde staining , three washings,1% bsa and I stain them with Cell membrane staining CellMask Deep...
15 July 2021 1,332 1 View
Hello scientific comunity. Does anyone know if there is a relationship between water contact angle and membrane configuration (dense, porous, and asymmetric)? Does the water contact angle suffer...
08 July 2021 6,633 5 View
Hello, I have a ~2 g frozen pellet of CHO cells overexpressing a membrane protein (stored at -80°C) which will be used for membrane vesicles preparation. I need to resuspend the pellet, split it...
05 July 2021 2,055 5 View
m-Phenylenediamine [1,3-diaminobenzene] solution I make for reverse osmosis membrane synthesis keeps changing colour, I suspect that some sort of reaction is happening that could affect the...
04 July 2021 162 4 View
To control bacterial growth and preserve Ultrafiltration and Reverse Osmosis membrane 1 wt.% sodium metabisulphite solution is used. is it possible to use this solution for the Nanofiltration...
18 June 2021 4,122 7 View
I'm researching the fusion of negative-sense RNA viruses with host membranes. However, I am unfamiliar with protein folding and unfolding, as well as conformational stability. I require further...
18 June 2021 3,565 1 View
I am an amateur vasp user.I have just started using vasp for some structural optimization calculations.I would like to know if there is some official mailing list for vasp?? So that I can post my...
16 June 2021 3,723 1 View
Hi everyone, I am working with cells labelled with Mcherry (membrane) and EGFP (cytoplasm). Following my experiment, I want to quantify the green signal which relocated to the membrane. How can I...
07 June 2021 1,726 3 View
It is in the literature that there are multiple Claudin proteins expressed in MDCK cells. Running the lysate in 50ul well SDS-PAGE, then transferring with BioRad Transblot system and traditional...
03 June 2021 5,927 3 View
I've heard that storage in TBST is acceptable. What specific temperature should I store at? How long is the membrane viable for?
03 June 2021 5,209 1 View
I've been having issues visualizing blots when probing for the Myc tag in HEK cells. I'll list my basic step outline that I follow, maybe that will be informative in picking out something I'm...
03 June 2021 9,217 3 View
Hi I would like to test a solid-state supercapacitor with a solid membrane, but I don't know how should I pack and seal it. Can I use a Swagelok cell? Also, what is the electrochemical test...
30 May 2021 5,931 4 View
Hi, I'm looking for a membrane dye that is compatible with EGFP and that can be excited with a 561 laser and observed with a 575-625 filter. The most important parameter is that it will not be a...
26 May 2021 7,467 2 View
I would like to determine if the novel drugs target peptidoglycan synthesis on gram-negative bacteria cells by staining with WGA stain, which stains the peptidoglycan layer. But the main problem...
25 May 2021 1,886 1 View
I want to know how I can prepare a composite membrane with uniform pores to separate sea water(water desalination).
25 May 2021 174 1 View