Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

I was genotyping some mice tail snips. It is a Cre Lox reporter mouse. The Cre is expressed using CRH promoter in the mouse brain and eGFP is the reporter gene. I isolated g-DNA and used it for...

12 January 2021 6,875 3 View

How can we increase awareness about Covid 19 prevention in our city among the local population?

The general public must be made aware of the mode of transmission, presenting symptoms and the measures that can be undertaken to prevent the spread of infection. Few options- Media, Webinars...

03 January 2021 3,538 13 View

How to remove rotation along z-axis and shift the origin to body's center?

I have attached the files with this post to explain me how to remove rotation and center the origin at the body's local coordinate. This is just one sample and I have 1000s of files like this. I...

18 December 2020 3,838 3 View

How can we know the exact soil taxonomy of any particular site. Is there any online available database for Indian soil taxonomy based on geography?

Please If one can let know the soil taxonomy for vindhyan region falling in Mirzapur district of Uttar Pradesh, India.

14 December 2020 9,879 14 View

Does anyone knows a tool (GOMS based or other) to automate calculation of task completion time?

Hello good human, really hope that you are doing well. I have been reading "The Psychology of Human Computer Interaction" by Stuart Card et al. They have introduced one of the ground breaking...

11 December 2020 7,269 3 View

Is there a solvent for polypropylene at room temperature?

I want to dissolve polypropylene for making a solution so as to make fibres at room temperature. I have tried toluene and xylene but without any success in dissolving.

06 December 2020 8,479 2 View

How to upload the name of notable journal The Business & Management Review (Print) when it is not showing in dropdown list?

I am also unable to upload notable journal The Business & Management Review (Print) with ISSN 2047-2854 (Print) ISSN 2047-2862 (ONLINE) Indexing with: ProQuest, Ebsco Host, EconBiz,...

24 June 2020 3,125 3 View

A code in EES is required for simulating ejector cycle?

How value of P_m will be calculated by iteration? All values on LHS of equation 13 and 14 are known P_m, h_mix, u_mix and v_mix are unknown quantities See equation 13,14,15,16 w is also...

01 May 2020 5,313 1 View

Does anyone know a good IHC protocol for FGFR1 detection on floating brain sections?

I am trying to standardize IHC for cFOS and FGFR1 detection on rat brain sections. I use 40 micron sections and have had good results for cFOS detection. But I have not had any positive results...

25 March 2020 582 5 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

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23 February 2021 4,014 3 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View