Hello,
I am currently doing a project related to cryo preservation and I need to ensure that the mitochondria in the cells preserved using my method stays intact (i.e., the mitochondrial integrity remains the same). Is it okay for me to use the presence and/or the quantity of the membrane surface proteins of these cells as biomarkers to determine whether the mitochondria inside these cells are intact?
Hello Michael Gitonobel
You can use the membrane integrity WB antibody cocktail Cat # ab 110414 from Abcam.
Please refer to the link below for more information.
https://www.abcam.com/membrane-integrity-wb-antibody-cocktail-ab110414.html
Best,
Malcolm Nobre Thank you very much for your answer! I appreciate it.
Hi!
If you want to show that mitochondria in cryopreserved cells are not only intact but also functional, you may consider adding some probes measuring mitochondrial health like flow cytometry / microscopy with MitoTracker dyes/JC-1 etc.
Good luck!
Charnoly body, Charnolophagy, Delta psi, 8-Hydroxy, 2-Deoxy Guanosine, 2,3- Dihydroxy Nonenal, Glutathione, LDH, SDH, SOD, Catalase, Ubiquinone-NADH Oxidoreductase (Complex-1), and many others. You may please refer to my books. The Charnoly Body and Charnolophagy in Amazon.com. Good luck with your research.
Respectfully,
Dean Sushil Sharma
Sushil Sharma Thank you very much for your recommendation, I'll take a look on your book. Good luck with your research too and stay safe.
You are most welcome Dr. Michael.
Respectfully,
Sushil Sharma, P. hD, D.M.R.l.T
Academic Dean, A.I.S.M.
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