Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures. | Contact experts in Membranes to get answers
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Questions related to Membranes
Hello, I have a protein which binds the plasma membrane of cells. I'd like to do directed evolution to make its membrane binding affinity stronger, but I'm having trouble with figuring out the...
07 October 2020 2,317 1 View
Procedure for isolation of Neisseria gonorrhoeae outer membrane vesicles as vaccine antigens.
02 October 2020 8,373 3 View
Hello. I need to detect a 100 KDa protein. I loaded 40 ug of samples in gel polyacrylamide 8%., SDS page in the mini gel-Biorad system, wet transfer (+20% methanol), 300 mA 1h. Nitrocellulose...
29 September 2020 1,111 8 View
If I wanted to stain for Hyaluronan within a tumour sample slide, what would be the best way to do this?
29 September 2020 7,519 12 View
Hi, I was wondering to know the effects of membrane shrinkage on the performance of PEMFCs? Can it affect the structural integrity of the assembly and interfaces? Best, Hossein
21 September 2020 4,667 4 View
i am working on gas separation based on flat sheet membranes. i was trying to measure the n2 permeability through the membranes and the value i am getting is very high. and the flow rate is also...
13 September 2020 8,764 11 View
HI, i am working gas separation using flat sheet membranes and I am new to this topic. I tried to find the permeability of nitrogen in the membrane by varying the inlet pressure and measuring the...
07 September 2020 4,945 3 View
I fabricated a cell using the swedgelok but I can't run any electrochemical test successfully. The CV goes to high current of about 1400mA and stops with the error c amplifier overload. The GCD...
16 August 2020 974 5 View
I am trying to detect DNA-protein covalent crosslinks which are induced by my drug treatment. I am following the RADAR assay protocol. Briefly, I am lysing cells using a buffer containing 6M GITC,...
31 July 2020 1,071 4 View
Keywords: Golgi membrane protein; mammlian system; overexpression is troublesome I'm currently on a project needs tons of a membrane protein on Golgi apparatus for structural analysis....
28 July 2020 760 1 View
Hi, I am trying to understand this figure from the article "Structural basis of membrane disruption and cellular toxicity by $\alpha$-synuclein oligomers" by Fusco et al. (2017) The figure...
16 July 2020 144 0 View
We are producing PEI membrane as support for gas separation membranes via phase inversion method by casting solution of PEI/NMP and water as coagulant. We cast on polyester support but membrane...
15 June 2020 2,410 1 View
Looking at the various CoV genomes, there are peptides/small proteins, aside from ORF1/2 on the genome, of which functions are unknown. These could circulate and in theory, once in abundance,...
26 March 2020 6,102 5 View
We want to measure whole whole-cell peak Na+ currents, isolated persistent and resurgent Na+ currents, and fEPSPs of Schaffer collateral synaptic responses in the CA1 of hippocampal slices. It was...
02 March 2020 6,656 0 View
Hi! I am looking for a dewatering method from an already quite concentrated solution at high temperatures. MD is usually applied at temperatures around 80°C and atmospheric pressure, but I have...
30 January 2020 1,865 1 View
I'm performing a Dot Blot and using Nylon Membrane stained with Methylene Blue for a loading control. Does anyone have any advice on how to image this membrane? I've tried using our Li-Cor Odyssey...
23 January 2020 2,265 2 View
As of recently I have been running quite a few western blots and my GAPDH results have been very inconsistent. Sometimes I get bands, other times I don't. The bands are not clear solid bands; they...
23 December 2019 5,747 3 View
dear all, I'm looking for a small-scale membrane for hydrogen separation from the air (or nitrogen). I need some information about available manufacturers. There is a lot of information about the...
16 December 2019 8,444 2 View
Hi everyone, I am looking for a protocol to isolate Endoplasmic Reticulum from tomato leaves to study fatty acid profile of its membrane. Thanks in advance
13 December 2019 1,680 0 View
Hello everyone, I am having problems with re-probing membranes for Western blot analysis. Almost every time I want to re-probe with either the same or a new primary antibody, I don't get any...
12 December 2019 5,152 5 View
Almost all membrane pore characterizing techniques cannot be used due to the mechanical instability of the flat membrane. I want to use deionized water flux (DI) and mathematical way to determine...
05 December 2019 3,952 2 View
I‘m an undergrad student working with ABC and MATE transporters and i was reading this paper http://www.plantcell.org/content/31/11/2789 bc they were using yeast microsomes for transporter assays...
05 December 2019 2,435 1 View
Hello, I am trying to optimize my northern hybridization. I realized that the membrane (SensiBlot Plus Nylon Membrane) that we are using has reached it's expiration date many years ago. Maybe...
04 December 2019 4,113 4 View
Already some research is conducted on GO,rGO for food packaging application. My point of concern is they haven't focused the after effects of release of GO,rGO in food in those papers. Is it...
27 November 2019 8,433 2 View