How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

How do I change a preprint to published?

I want to know how to change the status of an article.

21 February 2021 1,536 2 View

What buffer to use for biotinylation as alternative to PBS?

I'm looking to biotinylate a protein that precipitates in PBS. The recommended reconstitution buffer for the protein is urea/Tris buffer, pH 7,5 (7 M urea, 5 mM EDTA, 15 mM mercaptoethanol, 20 mM...

04 January 2021 7,681 5 View

Absolute Quantification: Serial dilution resulted in same CT values?

Hello, we are currently working on absolute quantification with SYBR Green qPCR. We excised from a plasmid containing the cDNA, a fragment, which will be amplified by the primers we used in our...

10 November 2020 769 4 View

What would cause Cell Titre Glo assay wells to luminesce less than the media only wells?

Hi all, I'm doing a bunch of Cell Titre Glo assays to assess the effects of several inhibitors on 2 cell lines. Problem is, for some of the wells they seem to be luminescing less than the...

30 October 2020 1,513 2 View

Can you recommend a reliable AMH ELISA for rat serum/plasma?

I am looking for a reliable and sensitive AMH ELISA for use with rat serum (and potentially plasma). Recommendations are welcomed! Thank you in advance.

08 July 2020 3,207 2 View

To what extend do you agree with this proposition on sustainable procurement??

" Picture a world where every product and appliance is environmentally friendly, where every supermarket item is fair trade, where corruption is an urban myth and poverty a long-distant memory....

19 May 2020 1,933 3 View

How Do You Incubate Microfluidic Chips with Cells?

Hello, I am interested in this research field. I have the prototype of my microfluidic chips, but I am having a hard time finding literature that says exactly how they incubate their chips (with...

21 April 2020 3,277 5 View

Meta-analysis on prognostic studies: How to reach an optimal cut-off value?

I'm doing a meta-analysis on prognostic studies. The studies I have included present the following values: AUC, 95% CI, a cut-off value and associated sensitivity and specificity. Also, is it...

01 March 2020 10,001 2 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View