A more relevant ratio would be that between the lysis buffer and the cell mass. That would depend, of course, on the composition of the lysis buffer (if you are counting on the lysis buffer itself to lyse the cells) or the method of cell lysis.
Since I use a French press to break open E. coli cells and the volume of the pressure cell is about 35 mL, I generally resuspend the pellet from 1 liter of culture in 30 mL of buffer.
Dear Mustafa Mansour
as Adam B Shapiro already told you generally the volume of the buffer is calculated in relation to the biomass amount and not culture volumes since the biomass can change a lot on the bassi of culture media that are you use and/or OD, time and temperature of induction.
In generall using sonication i'm using around 5-10ml for each g (wet biomass) while using chemical(enzimatic Lysis (eg CellLytic express) i'm using an high volume of buffers (10-20 ml for gram)
you can find some more information about CellLYtic express at the following link on my blog:
https://proteocool.blogspot.com/2021/08/tips-of-week11-chemicalenzymatic-cell.html
good luck
Manuele
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