What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
Thanks for your answer Jose.
Can we change USP method to In House method for carrying out the assay of any particular dosage form?
10 November 2017 2,743 1 View
If we increase or decrease the injection volume in HPLC will it make any difference in the retention time of the peak?
03 April 2016 2,373 2 View
Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?
03 April 2016 844 0 View
Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?
03 April 2016 3,735 0 View
Is there any material other than caffeine to calibrate the HPLC??
03 April 2016 7,365 4 View
What is the difference between moisture content and water content?
03 April 2016 4,478 4 View
Why do we use methanol as a solvent in KF standardisation? Why not other solvent?
03 April 2016 7,469 0 View
If any mobile phase contains a mixture of organic solvent and buffer, then for how many days can we store it for using it again and again? Also, if the mobile phase contains only the mixture of...
02 March 2016 8,022 8 View
Which is the best solvent used for washing of HPLC columns and in what concentration?
02 March 2016 9,490 6 View
I'm trying to develop assay method for Salmeterol and Fluticasone DPI, a product similar to Seroflo rotacaps, CIPLA. Has anyone worked or developed assay method for this product before? Please...
02 March 2016 1,478 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
31 July 2024 8,415 1 View