Why do Methanol gives broad peak shape as compare to Acetonitrile in Liquid Chromatography ??

05 May 2016 1 938 Report

In case of liquid chromatography when methanol is used as organic modifier it gives broad peak shape in comparison to Acetonitrile. Even though the buffer ratio may remain same for the both.

Stefano Polesello

look at this http://www.shimadzu.com/an/hplc/support/lib/lctalk/35/35lab.html

it depends also on the type of compounds to be separated. In some cases, when a hydrogen bond can be established with methanol, the effect is the opposite

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