I'm using commercial Herceptin for some experiments and when running FPLC (column superdex 200, PBS mobile phase, eluting 0.5ml/min) on my samples I find, besides the antibody peak (UV 280nm) at 26-27 minutes, a peak at around 36-37 minutes (corresponding to a mass around 10 000, i think). Anyone else who have had the same problem and might know what this contamination could be?
It will depend on whose Herceptin preparation you are using. Some contain Chinese Hamster Ovary (CHO) cell proteins. Look in the contraindication info as it may tell you what other components are present if it is not CHO protein(s)
And if you want it pure, you could take fractions discarding the contaminant and check if it's affecting your experiments.
Dear Professor Aneheim
We bought Herceptin (a vial of powder for concentrate for solution for infusion) from Roche Ltd. (Switzerland). We dialyzed it against PBS buffer and we did not observe any contamination in FPLC. I think the opinion of Dr. Worrall is correct and in this case you can use the method described by Dr. Jover or you can buy Herceptin from Roche Company like us.
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