Having just looked at the structure, you should be able to analyse it by HPLC, but you'd need a non-UV detector, or perform some sort of derivatisation to make it detectable. It would not give you a good UV response without chemical modification, so detection limits would be high.

But that's only part of the question really... what else is likely to be in the sample submitted for analysis? Your HPLC method will need to separate muramic acid from everything else giving a detector response. GC typically gives higher resolution separations than HPLC.

Thank u for your answer

try this paper.

Appl Environ Microbiol. 1985 Aug;50(2):229-37.

Muramic Acid measurements for bacterial investigations in marine environments by high-pressure liquid chromatography.

Mimura T1, Romano JC.

Author information

Abstract

Muramic acid, a constituent of procaryotic cell walls, was assayed by high-pressure liquid chromatography in samples from several marine environments (water column, surface microlayer, and sediment) and a bacterial culture. It is used as a microbial biomass indicator. The method gave a good separation of muramic acid from interfering compounds with satisfactory reproducibility. A pseudomonad culture had a muramic acid content of 4.7 x 10 to 5.3 x 10 mug per cell during growth. In natural water samples, highly significant relationships were found between muramic acid concentrations and bacterial numbers for populations of 10 to 10 cells per liter. The muramic acid content in natural marine water decreased from 5.3 x 10 to 1.6 x 10 mug per cell with increasing depth. In coastal sediments exposed to sewage pollution, concentrations of muramic acid, ATP, organic carbon, and total amino acids displayed a parallel decrease with increasing distance from the sewage outlet. Advantages of muramic acid measurement by high-pressure liquid chromatography are its high sensitivity and reduction of preparation steps, allowing a short time analysis