I have followed the journal that I refer but the solution I prepared to dissolve and dilute my standards was clear at the beginning. After 2 to 3 minutes it started to be cloudy. Can anyone can advise me on this case?
If you cannot dissolve it by sonicating, spin it down and inject the supernatant.
Do not inject the "cloudy" solution. It will block your column.
I agree with the answers above. Cloudy solution will damage the costly column. First spin the sample, take the supernatant, if it is not clear yet..then filter it with the membrane filter. I hope you would get a clear solution then.
before injection Sonicate the solution then filter it by using Syringe filter make sure the Solution should not be colored
if any cloudy or coloured solution it will affect your column & check valves
In the examination of environmental probes from areas, which are contaminated with explosives, more often cloudy solutions appeared. Therefore I am using a little pre-column, which I can put in the waste.
You can do centrifugation process and microfiltration through 0.45 micron filter.
I agree with the above, but it is also good to contact the authors of the article you are referring to, and ask them if they had similar problems (and how they fixed it). Sure, you can remove the cloud, but you never know what you removed when you did that so your entire analysis becomes less valid.
Before inject you may filter the solution with 0.45 cellulose membrane. Or if possible then sonicate the solution if it is turbid. After sonication particles may dissolve and clear solution obtained.
Will put at risk the HPLC equipment, one of the conditions of use is that all solutions must be filtered ...
During preparation of samples for analysis,may results in the formation of a cloudy white solution. When this occurs, the tubes are centrifuged at 10 000 rev/min for 10 min and the resulting clear supernatants are taken for analysis.
it is better not to inject that kind of sample to the column because as Rajesh said it may be clog the column and also cause memory effect.
thank u for your suggestions..I did contact the author but still do not get any answer. But maybe I should try centrifuge or heat the solution before dissolve the standards. I did filtered with 0.27 um membrane but after sometime it start to cloudy again.
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