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Questions related to Laboratory Techniques
In most cases, if X-ray shows standby state, when we measure PXRD pattern of the sample, the X-ray automatically changes to on state and shows voltage and current value. This time, when we started...
16 October 2014 5,601 1 View
Actually the reading or the ct values become unchanged after few dilutions I don't understand although the bands on gel are quite prominent with all dilutions. It means reaction is already...
10 October 2014 1,260 1 View
I need to defrost a -80 freezer with competent cells and other laboratory reagents in it. Any suggestions on the best way to do so are welcome!
10 October 2014 4,809 1 View
10 October 2014 8,084 1 View
I want to isolate the membrane proteins from bacteria, I reviewed many articles, they have used 100000 g for separation. I don't have the ultrahigh centrifuge, we have maximum of 10000 g can...
10 October 2014 9,895 5 View
I'm kind of confused with data normalization in CLC, I Compared RNA-seq results after doing normalization in data or on an experiment using legacy version and also using original data and...
10 October 2014 796 6 View
I am doing my research on Sero-mapping and molecular characterization of Brucella species in ruminants, but I could not get a specific molecular technique which differentiates each species and...
10 September 2014 3,886 2 View
back from the summer holidays a doubt has arisen.Do I have to sterilize 1.5 Ml PLASTIC tubes (the eppendorff) and I have some glass jars with lid (jam jars).I have to put them in the autoclave...
09 September 2014 2,110 3 View
The purified natural extract when dried with vacuum oven becomes dark in colour whereas the same extract when freeze dried gives colorless product. Even though the vacuum oven drying was carried...
09 September 2014 3,526 4 View
09 September 2014 1,316 2 View
I am undertaking immunohistochemistry on sections from a knockout mouse line we have. The issue I am having is that in the final steps of the protocol, a number of ethanol baths (50%, 70%, 95%,...
09 September 2014 776 3 View
I need to calculate the energy required for evaporating a solvent mixture. How can I do that? Does the volume (it's going to be evaporation of a droplet not a bulky solution) having an effect on...
09 September 2014 6,289 2 View
Hi I hope someone can be of assistance. I want to stain whole Arabidopsis rosettes for starch content. The staining itself is not the problem, the ‘assembly’ of the rosettes are. When it is...
09 September 2014 2,600 3 View
For example if we have L-alanine and we want to convert it to L-alanine monohydrochloride, we have to add 4 M (as a concentration) of HCl acid. Why 4M no more or less?
09 September 2014 9,118 1 View
What is the exact downstream process followed after microbial synthesis?
09 September 2014 4,784 1 View
Our heating furnace is not a vacuum furnace. Any way to keep pure magnesium from being oxidized in high temperature (600 degree)? Any idea except buying a vacuum furnace?
28 August 2014 3,898 2 View
I need to clean the substrate without any chemical impurities in order to get a highly quality of deposited thin film.
26 August 2014 2,779 10 View
I'm looking for a protocol to isolate RNA from young, as well mature, seeds of wheat.
25 August 2014 3,534 6 View
I do need a thin film of hydrogel for TEM characterization.
25 August 2014 2,959 9 View
I wonder if there is a method that can be used for labs that do not have easy access to liquid nitrogen or dry ice to snap-freeze tissue samples? The temperature should reach at least -20°C,...
25 August 2014 6,110 2 View
I used Tyrosine to do a standard curve for protase enzyme test, and it was insoluble in water
08 August 2014 5,480 5 View
I've been working with bone marrow dendritic cells differentiated with r-GMCSF. After differentiation, I plate the cells and after 2 hours they are completely adhered. In my experimental protocol,...
08 August 2014 4,144 16 View
Hi guys, So while carrying out PAGE, following the addition of propanol alcohol, the surface becomes smooth, but not straight, it usually starts to take a lower angle near the end. It's not a huge...
08 August 2014 1,896 13 View
I have tried water in which extract is insoluble and DMSO & other organic solvent itself is showing inhibitory effect in the assay.
08 August 2014 1,547 2 View