Is there an alternative to liquid-nitrogen snap-freezing?

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Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

Can anyone give me a guide regarding vcf files?

I have a VCF file with SNPs using the IGV program, when uploading the file some SNPs that are observed in a particular gene in IGV, when verifying it in GDV or Ensembl do not match

10 August 2020 699 3 View

How to download citation network data from Web of Science (WoS)?

I want to do citation analysis on papers on ecnomics, but I don't know exactly how to get this data from Web of Science, though many sources say this is one of the very good datasets in this area....

16 March 2020 4,184 4 View

What is a good dataset and technique for citation analysis in Economics?

I would like to track the presence of some ideas in papers in economics, and I have been reading about citation network analysis and I have found some datasets (like the...

26 February 2020 2,848 1 View

Can someone explain about Different output of C/T vs A/G CSN1S2?

A NGS panel of truseq was performed, the SNP expected of CSN1S2, Ch 6, Position 87266177 are C/T, but we received A/G. There is an explain about that?

28 January 2020 1,118 2 View

Can you recommand Fc block antibody to use for immunophenotyping of rat stem cells (FACS)?

Dear RG community, I am looking for anti-rat FC blocking antibodies (CD16/C32) for the QC of a FACS immunophenotyping experiment. So far, I can only find anti-mouse Fc block from manufacturers...

04 December 2019 7,336 1 View

What are overall assumptions for comparing two enzymes of the same class consisting of more or less different amino acids?

I do research on genetic code and aminoacyl-tRNA-synthetase (aaRS) evolution. An aaRS consists eihter of 18, 19 or 20 amino acids. Are there biological rules or findings that allow the assumption...

13 February 2019 5,734 7 View

Which simulations of genetic code evolution do exist?

Are there simulations that... - simulate changes in the tRNA pool? - simulate flexizymes? - simulate aaRS? I did a lot of research but didn't find practical approachs on the role of these three...

22 January 2019 8,768 1 View

How long does an Aminoacyl-tRNA-Synthetase live? How often is it reproduced?

For my current project I do a lot of research about aaRS but didn't find answers to these questions yet. I am happy about any information about the lifecycle of these enzymes.

05 November 2018 2,831 2 View

Where i can get the software ASSIGN 400ATF?

Can someone help me how i get the Assign 400ATF software for analysis of sequence of BoLA-DRB3 gene?

06 November 2017 7,938 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View