I wonder if there is a method that can be used for labs that do not have easy access to liquid nitrogen or dry ice to snap-freeze tissue samples? The temperature should reach at least -20°C, better would be -80°C and lower.
RWC was calculated according to Dhopte and Manuel (2002):
RWC = (FW-DW/TW-DW) ×100
Where, FW is fresh weight, DW is dry weight and TW is turger weight of leaf samples.
Products can be used depending on the Dhopte
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