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Questions related to Laboratory Techniques
Temperature i want to keep formalin is 10 degreeC for 1 year
03 March 2016 711 0 View
Class II, type A2 cabinets are widely use in tissue culture but are not meant to handle toxic, volatile or flammable chemicals.
03 March 2016 3,619 3 View
01 March 2016 8,978 4 View
I would like to do some study using our human sweat. But the sample collection is not enough for me. So I have decided to do it by my own.
23 February 2016 1,114 2 View
We are basically looking for an ELN thats freely available and is user friendle so that even novices to the ELn environment will adapt to it easily.
18 February 2016 9,327 5 View
I want to derivatize my fatty acids in methyl esters. according to protocol I should add 10% BF3/methanol in the sample. I want to confirm that does 10% BF3/methanl means 10 ml BF3 in 90ml...
03 February 2016 4,365 3 View
It is a very common procedure to transcardially perfuse mice and rats with 4% PFA, and then dissect out the tissues. Formaldehyde (and PFA) is a known carcinogen, and all the safety material says...
02 February 2016 8,816 2 View
02 February 2016 4,429 2 View
02 February 2016 3,248 5 View
i want to preserve my bacterial strains for 2 years . dose glycerol broth is best for it? can anyone send me best method for preparation of glycerol broth and preservation of bacterial strain?
02 February 2016 7,935 2 View
Dear EPR community, I'd like to know a reliable method to clean out EPR tubes which previously held a sample in solution, particularly in the case of samples containing transition metal species....
02 February 2016 1,365 3 View
In my research it is required to label T4 DNA Ligase with Rhodamine B, is there anyone who recommend a low cost protocol for labeling.
02 February 2016 5,781 0 View
02 February 2016 2,642 3 View
Hi I am trying to make gelatin methacrylate and I've reached the final step of freeze drying it. There seems to be a lot of protocols for freeze drying with varying temperature and pressure...
05 January 2016 6,103 3 View
01 January 2016 3,348 4 View
Does anyone had experiences for Bioremediation of MTBE with bacteria? What were your experiences? Thanks
23 December 2015 1,146 6 View
I need to clean glass vials used in high pressure reactor for catalysis. Aqua regia and liquid detergent does not work much.
12 December 2015 2,672 3 View
I have been shipping primer aliquots (10 pmol/ul) but they seem to be not working after about 10 days in transit. Thus, could I maybe dry them for shipping?
12 December 2015 6,738 2 View
If you are using disinfectant into waterbath, can you let me know what is the brand and chemical name of it. Do you just use CuSO4 or bleach into waterbath? Thanks.
12 December 2015 1,563 0 View
Hi, I am trying to estimate the operational costs of a GC/MS, including all consumables. I understand that this depends on the frequency of use, types and nature of samples being analysed, etc....
12 December 2015 2,973 6 View
I am growing biofilms in 96 well plates. Currently, I'm using pipette tips to scrape the films, and I then aspirate, vortex, and culture to measure CFUs. I'd like some easier and more uniform way...
12 December 2015 9,448 4 View
12 December 2015 630 7 View
Hi all. Our Autochem TPD instrument is facing one kind of problem as you can see in attachment baseline is not getting stable. What are the remedies for that? On the right side analysis taken for...
12 December 2015 10,017 3 View
10 December 2015 8,637 5 View