Laboratory Techniques

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Questions related to Laboratory Techniques

How do I prepare benzonase buffer?

I need to prepare benzonase buffer with Tris-HCl and MgCl2(H2O)6. What concentration would be considered appropriate for activating benzonase?

08 December 2015 6,131 5 View

Remove Coverslip on Glass Bottom Dishes?

Does anyone know how to make the fluid to remove the coverslip of glass bottom dishes?

11 November 2015 2,193 0 View

The MTT assay of chicken line fibroblast showed no absorbance though the assay was carried on healthy cell culture. What is the reason for this?

Does any one know the reason behind the non-absorbance during MTT ASSAY even though the assay was carried out on the healthy fibroblastic cell culture. Are there any ways by which we can check the...

03 October 2015 7,840 3 View

Has anyone developed a protocol for the collection of secretory proteins from whole tissue without tissue homogenization and cell lysis?

I am hoping to collect a secretory protein from fresh and flash frozen breast tissue for analysis by proximity ligation assay. Specifically, I am in need of a protocol which would allow me to...

26 August 2015 1,263 5 View

Is it possible to wash Millipore centrifuge tube from noble metal sediment?

We are using milipore centrifuge Filter tube (100 kDa) to concentrate gold nanoparticle. but after using them, gold debris will be funded in the tube and we try to use centrifuge tube again...

08 August 2015 4,756 0 View

Is meta-phosphoric acid (s) stable enough for 1 month to be dissolved in de-ionised water and kept at -20degC in a centrifuge tube?

Breaking the meta-phosphoric acid crystals with a mortar and pestle, weighing, and then making sure all dissolves in water could be time consuming. Is it possible to prepare a large batch of the...

21 July 2015 4,703 1 View

Can anyone recommend a CO2 container for killing spider mites?

Hello! I'm trying to kill spider mites with CO2.Does someone has a clue about what type of container I can use so the gas do not leak?

07 July 2015 9,948 1 View

Is meta-phosphoric acid (s) stable enough for 1 month to be dissolved in de-ionised water and kept at -20degC in a centrifuge tube?

Breaking the meta-phosphoric acid crystals with a mortar and pestle, weighing, and then making sure all dissolves in water could be time consuming. Is it possible to prepare a large batch of the...

07 July 2015 5,839 1 View

How can I avoid degradation of compound by water during RP-HPLC analysis?

I observe partial degradation of polar compound by water during RP-HPLC analysis. Does anyone have suggestions for RP-HPLC methods were this can be avoided. HILIC and NP have been examined. The...

07 July 2015 1,777 6 View

How can I avoid degradation of compound by water during RP-HPLC analysis?

I observe partial degradation of polar compound by water during RP-HPLC analysis. Does anyone have suggestions for RP-HPLC methods were this can be avoided. HILIC and NP have been examined. The...

01 July 2015 9,798 5 View

What type of syringe filter is most suitable for pectin solutions?

More specifically for 10 mL of a 0.025% (w/v) solution of LMP

06 June 2015 1,956 2 View

Does the Ghrelin value depend on laboratory technique?

Dear fellow researchers, I'm currently conducting a systematic review on the effects of bariatric surgery on Ghrelin levels. In order to stratify my groups for a meta-analysis I need to identify...

06 June 2015 2,338 7 View

Does anyone have a tutorial on SPICE for flow cytometry?

I'm trying to start using SPICE to analyze flow cytometry data and I'm kind of lost. Any help would be appreciated!!! Thanks

05 May 2015 5,599 5 View

How to estimate the titer of adenoviruses collected from a 10 cm dish?

I saw a website that the estimated titers of adenoviruses in the supernatant snd pellet collected from a 10 cm dish. The information is very useful, but I couldn't find this website again. Would...

10 April 2015 9,770 2 View

How can I stir very small volumes (down to 200uL) with stirrer?

We need to stir a 200-300uL volume in a microtube, and currently are relying on flea stirrers; however, even the smallest stirrer we can find always needs cut with bolt cutters to get a small...

04 April 2015 9,277 6 View

How to estimate the titer of adenoviruses collected from a 10 cm dish?

I saw a website that the estimated titers of adenoviruses in the supernatant snd pellet collected from a 10 cm dish. The information is very useful, but I couldn't find this website again. Would...

04 April 2015 2,909 2 View

How can I verify the calibration of a centrifuge?

I need a detailed procedure for verification of a calibrated centrifuge.

18 March 2015 2,236 8 View

How can I separate a Vacuum filtration unit that is stuck?

Dear colleagues.I have a simple filtration unit like this http://pusatalatlab.com/?ms=9&idd=243/vacuum%20filtration%20unit%20for%20membranes Now I have the problem that the kitasato has joined...

11 March 2015 8,810 2 View

Can you suggest some useful books on Laboratory Techniques in Environmental Engineering?

Please share some useful books on the Laboratory Techniques in Environmental Engineering. Measurements of solids, pH, alkalinity, hardness, DO, BOD, COD, SVI, turbidity, MPN, chlorine residual,...

03 March 2015 4,767 6 View

How can I separate a Vacuum filtration unit that is stuck?

Dear colleagues.I have a simple filtration unit like this http://pusatalatlab.com/?ms=9&idd=243/vacuum%20filtration%20unit%20for%20membranes Now I have the problem that the kitasato has joined...

03 March 2015 4,188 3 View

How can I verify the calibration of a centrifuge?

I need a detailed procedure for verification of a calibrated centrifuge.

03 March 2015 1,957 9 View

What are some good devices to effectively and quickly mince human adipose tissue?

We routinely get between 50 and 150 grams of adipose tissue that we process for stromal vascular cells - we currently mince by hand with scissors, but it's a huge time drain and takes a long time,...

12 February 2015 10,009 11 View

There is any method for convert CuCl2:2H2O to CuCl2 only?

I want to prepare cucl2 from its dihydrated form, is there any method available?

07 February 2015 4,414 3 View

What is the purpose of very long time stirring and of ageing a material?

I have read my articles in which researchers do very long time stirring up to 12 hours stirring. So I want to know what is physics or chemistry behind such long time stirring? Also I have seen...

02 February 2015 570 5 View

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