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Questions related to Laboratory Techniques
The DNA extraction in the lab became a bottleneck. We are considering buying equipment for 96-plate high throughput (vacuum pump, manifold, etc) or outsourcing to a company. Does anyone have a...
03 March 2017 4,710 0 View
Our biochemistry lab received a dishwasher from an agriculture lab. We were wondering if there are any recommendations on how to clean the dishwasher before we use it ourselves.I'm assuming we...
03 March 2017 1,122 1 View
I have found a mold contamination in our cell culture flasks. After some investigation I have discovered that a laminar flow hood is contaminated with mold spores! Have anyone had such experience...
03 March 2017 857 1 View
01 March 2017 6,033 1 View
Any other recommendable methods? Is it alright just to leave the alcohol immersed plant extract to open air with the containers lid open for the evaporation?
26 February 2017 8,574 3 View
Anybody know where you can get bottles for a Kompspin KA-9 rotor (4 1 l bottles)?
10 February 2017 4,709 1 View
Thermo Scientific is the supplier of the Sorvall Legend Microcentrifuge 21 Thanks
02 February 2017 9,783 1 View
We have a Beckman L7-65 ultracentrifuge that is nearly 30 years old. Can it be certified as safe for use with new rotors?
02 February 2017 8,948 1 View
02 February 2017 7,232 1 View
Presumably this is only for low-vac? I cannot find appropriate information through the web. My hood has a valve for that. Problem is we got it as a gift while ago and none asked for any...
02 February 2017 3,315 1 View
02 February 2017 3,779 3 View
01 February 2017 6,986 1 View
Stains can be removed from concrete with dry or mechanical methods, or by wet methods using chemical or water. Common dry methods include sandblasting, flame cleaning and shot blasting, grinding,...
17 January 2017 5,316 1 View
My tubes became unwashable from something organic. Invisible by naked eye, but the signal remained (some stable radicals) I tryed with acetone, dichlormethane, hexane, chloroform, mixure of acids...
01 January 2017 7,555 1 View
I have prepared a stock solution of deoxycholate 10% to used in pull downs, however, I was wondering for how long could I keep it? and in which conditions must be stored that solution?
01 January 2017 4,605 1 View
Dear Colleagues, On our Waters Xevo TQS MS (installed in mid2015), the Ion Guide Pirani is already higher than usual when the cone valve is closed (5*10-4), and after opening, the error message...
22 December 2016 673 1 View
I'm planning to do an experiment in my lab (cell culturing) and the problem is that my lab does not a hood for for proper sterilization. Is there any possible was to perform lab work without using...
12 December 2016 6,055 3 View
12 December 2016 9,049 1 View
09 December 2016 5,952 2 View
A matrix of CdS/PMMA was prepared by trying to dissolve PMMA in chloroform. But PMMA did not dissolve in the solvent and after keeping the solution as such , the PMMA has stuck to the walls of the...
11 November 2016 2,087 2 View
I just purchased an ampule with 10 ml of RuO4 0.5% in water solution. Acros 389000100 (CAS: 20427-56-9) Ins How should it be handled after breaking the ampule? Should I dilute it more? Could it be...
11 November 2016 815 0 View
I run a Resin Infusion procedure. I use a vacuum pump so that I can reach the vacuum pressure. Can I adjust the pressure by turning the hand valve even if the pump is allowed to operate only at...
11 November 2016 2,791 0 View
If I resuspend dihydrorhodamine 123 in EtOH, is it best to store aliquots in -20 or in liquid N2? Also I've seen one recommendation not to use a reconstituted aliquot beyond 1 month. What's the...
11 November 2016 2,673 4 View
Hello, I know this question is not real science but we all fight during our days with the equipment in the lab. We defrosted our -80C freezer (Brunswick) and replace the gasket because it wasn't...
11 November 2016 5,909 1 View