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Questions related to Laboratory Techniques
I want to use dry ice as a coolant , how can i use it so that its flow can also be controlled in order to get the desired temperature.
02 February 2015 4,822 1 View
Does anyone know if a Corning 24-well tissue culture plate can be heated to 37-degrees C (98.6-degrees F) without any issues? The last thing I want is the plate to melt, or some other catastrophic...
02 February 2015 8,203 3 View
I want to prepare cucl2 from its dihydrated form, is there any method available?
02 February 2015 4,770 3 View
We routinely get between 50 and 150 grams of adipose tissue that we process for stromal vascular cells - we currently mince by hand with scissors, but it's a huge time drain and takes a long time,...
02 February 2015 1,226 12 View
Hi, I'm looking for a protocol for analyzing my bright field images stained with DAB and hematoxylin counterstain. I want to take into consideration the background DAB stain and my true DAB signal...
29 January 2015 4,753 12 View
01 January 2015 2,140 13 View
I have done sampling of PM10 in ambient air with the help of respirable dust sampler.Please provide sampling extraction technique for further analysis.
12 December 2014 950 1 View
Previous when I did the qPCR with Bio-rad iCycler iQ™ Real-Time PCR Detection System, I can get the PCR efficiency between 95%~105%. Recently, our lab introduced the Bio-rad CFX 96. However, when...
12 December 2014 6,340 5 View
Often when I carry a homogeneous solution and put into culture medium (DMEM supplemented as fetal bovine serum) the solution becomes cloudy and forms a pellet when centrifugal . Anyone have any...
12 December 2014 2,811 4 View
11 December 2014 960 1 View
I will do DNA isolation from tap water sample, I am wondering how many water sample do I need? Some papers stated between 1-10 liter would be enough. Thank you.
11 November 2014 6,868 4 View
Sample of around 100ml in NaOH at 0.5M
11 November 2014 6,616 4 View
I would like to know whether strong acids, like 99-100% sulfuric acid, 65% nitric acid or 98-100% formic acid, stored for many years (lets say over 20 years) deteriorate/change in such a way that...
11 November 2014 8,263 3 View
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11 November 2014 5,079 0 View
I extracted whole blood samples using the Qiagen DNeasy blood and tissue kit. When quantifying the DNA on the Qubit, I get baffling low DNA concentrations. Is there something that I might be doing...
11 November 2014 6,330 5 View
Which auto-clustering software or package is most suitable for flow cytometry analysis? FlowMeans, FlowDensity or other soft?
11 November 2014 5,855 2 View
1- Another project is going on in the same instrument (to measure amino acid). Only the column is different. This one's result looks fine. 2- Every time, the first sample has good signal, then the...
11 November 2014 4,010 6 View
With regard to the same, I have found that glybenclamide dissolves well in DMSO, however, when I dilute the stock solution into a working solution with either PBS or water, the solution will give...
11 November 2014 7,829 0 View
Our microscope-integrated incubator is not working properly. Can we add some carbon tabs to the culture wells to control the CO2 level during imaging? Where can we obtain those tablets? Thanks
11 November 2014 2,357 5 View
I know for example that polypropylene materials cannot be used due to their affinity and binding with endotoxins. Can Polystyren materials be used or is it better to exclusively use glass material?
11 November 2014 5,955 0 View
I am trying to increase the adhesiveness of polystyrene using 3-glycidoxypropyltrimethoxysilane, but do not either have a good protocol or dont have the right silane. Can anyone help please.
11 November 2014 10,175 3 View
Hello everyone,I would like to perform a purification (fractionation) of tubulin from cell lines in order to study the effect of some tubulin binding agents. I found a protocol explaining how to...
11 November 2014 2,523 1 View
10 November 2014 2,441 1 View
06 November 2014 4,389 3 View