Hi
I hope someone can be of assistance. I want to stain whole Arabidopsis rosettes for starch content. The staining itself is not the problem, the ‘assembly’ of the rosettes are. When it is finished de-coloring in ethanol and stained in Lugol solution, getting it back to any shape that resembles a nice rosette is near impossible. I spend quite a while trying to get my rosette to look like a rosette.
I have seen some papers that have stained the whole rosette, just not sure how they managed to ‘assemble’ it.
Thank you in advance!
I attached a picture of my attempt at 'assembling' my stained rosette (end of night).
In case you need to know the staining process:
75% EtOH microwave for 10s (repeat twice) - wash with mQH2O - stain in Lugol solution 2 min - wash mQH2O
I just saw your question. If it is not too late, you can have a try of what I have done.
I suspend the staining rosette in water or any transparent solution in a plate or beaker. It is easy to adjust the leave position and the whole rosette will maintain a natural pose. Then you can take a good picture from it.
It will even better if you have a water proof lens.
Good luck.
Xiang Ming
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