What is the best way to defrost a -80 freezer?

10 October 2014 1 5K Report

I need to defrost a -80 freezer with competent cells and other laboratory reagents in it. Any suggestions on the best way to do so are welcome!

Antonio La Gioia

I assume that the material that must be defrosted it is in small volumes. I suggest that you follow a descending sequence of temperatures, depending on the available freezers: -50/35; -20/10; -4; +4; room. To speed up the steps you can prepare a series of small containers of ethyl alcohol kept for different times to -80° C (freezing point -117). Using short sequential dive, the temperature of your samples can be increased gradually and without thermal shock.

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