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Questions related to Laboratory Techniques
I extracted bacterial prodigiosin by using acetone. Is its activity or stability loss when stored in 4 degree ? Please suggest a proper way of storage? Now I am storing it in 4 degree.
08 August 2016 7,441 0 View
Dear colleagues I need the help for the antiurolithiatic activity, first I have three questions: 1- My problem is in choosing the standard, Cystone, it is not sold by Sigma Aldrich, Can I use...
05 August 2016 2,581 1 View
A chichibabin reaction of aromatic aldehyde with aromatic ketone and ammonium acetate was charged in acetic acid. Upon completion of reaction, it was washed with cold water. After filteration, the...
03 August 2016 7,876 11 View
I bought a new LCMS column that is very long as I require of a very good analyte separation. Just realised the column doesn't physically fit inside the LCMS instrument slot but I would swear I've...
30 July 2016 3,095 3 View
Besides use of ethanol, is there any other alternative medium to which fecal samples can be stored before transport to the laboratory for analysis? transport medium for fecal samples for stability...
17 July 2016 9,249 1 View
i want to see the possibility of applying zero energy cooling in a very humid environment
07 July 2016 5,907 1 View
I need to transfer the flask to another lab ten minutes away to take pictures as my lab not have computer connected microscope and I failed to take pic using phone. The whole process may take one...
07 July 2016 4,743 0 View
The terrarium should allow flow of oxygen without allowing the insects to escape. It should also be big enough to house a small amount of food and/or leaves.
07 July 2016 4,932 4 View
I am trying to freeze-dry samples made of 0.1mL water and 0.5mL methanol. My freeze-drier only reaches -55C and methanol freezes at about -98C so the samples are never actually frozen. The vaccum...
07 July 2016 8,990 1 View
when i make a film, i usually use to vaccum filteration but, i have difficult to peel off film at the filter. and memebrane is 'Nylon Menbrane Filters 0.2um 0.47nm' anyone who has clue, please...
07 July 2016 3,298 1 View
07 July 2016 1,312 1 View
I found a protocol to do Normal Goat Serum, but I am not sure if it is correct. Is it needed to make a heat treatment? It is for using in IHQ as blocking serum. Can anybody help me,...
07 July 2016 9,319 4 View
07 July 2016 6,034 3 View
In the Article: An Approach to Combining Results from Multiple Methods Motivated by the ISO GUM, where did the value of u(S1) come from and how was it calculated? Is it just S1(X) / sqrt of n1...
07 July 2016 8,361 0 View
I have used NaH for a synthesis. It was dispersed in mineral oil. How can I remove the mineral oil completely from my product?
07 July 2016 9,624 4 View
Hello, I have a DC diaphragm water pump with 4 liter/min flow rate and maximum 100 PSI pressure. I plan to use this pump in a desalination system. I will need to the flow rates between 0-3...
07 July 2016 2,749 2 View
Hi all I am now working in an analytical lab with ~40yrs history, some of the chemicals are expired for more than 10 yrs, should I be using them? And wil it affect the results I get from them?...
23 June 2016 5,479 3 View
I see some people pour the exhausted medium into a beaker which contains bleach placed inside the hood instead of suction. The idea seems good saving resources but was wondering if bleach can...
06 June 2016 7,323 0 View
C. elegans normally swim on their side but to get images of the cell bodies I want, they need to be fixed on their backs. How should I go about this process?
06 June 2016 7,053 1 View
Does anyone have any recommendations for cell scrapers? We have been using Fisher's 39 cm scraper: https://www.fishersci.com/shop/products/fisherbrand-cell-scrapers-3/p-3821025 The end tends to...
06 June 2016 8,125 0 View
Hi, I'm running an experiment that requires ultra-clean microscope slides (think darkfield microscopy), and was wondering if anyone had a suggested approach for cleaning slides of particles and...
06 June 2016 6,152 5 View
I ball milled a mixture of ethanol,ethylene glyco and yttrium ytterbium oxide. I used rotary evaporator to dry this mixture but it is not drying.Please who can help me out? Ethylene glyco was used...
06 June 2016 1,041 2 View
Hi, I just want to make sure the glass wool in our lab is free of most common contaminants. Is there a protocol that I can follow to clean the glass wool? Should I just use acetone or water? Thanks!!
06 June 2016 2,771 1 View
I have reacted my compound with tetrabutylammoniumhydroxide. now I want to remove excess of tetrabutylammoniumhydroxide but both my compound and tetrabutylammoniumhydroxide soluble in organic...
06 June 2016 8,492 4 View