Can thioglycolate used in recruit peritoneal macrophage pass through 0.22 um filter for sterilization?

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David Fast

Do you have access to an autoclave? That is the standard method for sterilizing thioglycollate. Also, if you can let it sit for up to 2 months following autoclaving you'll get better yield of cells.

Hector Pons

Even a presure cooker will do the job, one hour at 15 psi, let cool slow to avoid ebullition