In the literature, a often used concentration is 10 µg/ml.

We've tested nivolumab and found activity at all concentrations tested.  We did half log steps between 0.1 ug/mL and 10 ug/mL.

I would recommend that you empirically determine the optimum concentration for your particular assay by performing a dose-titration curve. You should do this a a couple of your favorite donors so you know the variability.

Good point Sandra.  Different antibodies, different donors, culture medium, always good to test a range of concentrations in your own lab.

BTW, good to see your name pop up.  Hope all is well with you.

Anne can you shortly tell us more about the "activity" that you observed? What was the experimental set-up?