I am using EDC/NHS/MES buffers but the antibody I want to conjugate is really expensive so I am trying to optimise the protocol using BSA.
The protocol I have been using is to add 10mg of NPs with 2mg protein but the max yield this protocol gives is 2.2mg. To me this is not acceptable and a really inefficient protocol.
Any and all advice appreciated.
Thank you
Dear Amanda, were you able to optimize your protocol? Are there other methods to purify PLGA-NPs in place of centrifuge?
Hi Filippo, apparently Dialysis is a good alternative used by many researchers optimising nanoparticle purification steps although I haven't tried it myself. I find centrifugation of PLGA particles at ~30000rpm for 45mins at 4C sufficient. I then use a sonication bath to re-suspend the particles from the pellet. Hope this helps.
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