The ATCC requires a Bio-safety Level 2 to work with certain species of this organism. The best thing would be to consult your Institution's Bio-safety Officer immediately BEFORE starting ANY research work in a lab that is not certified to work with BSL-2 agents.
Should be adopted to good laboratory practices, including universal precautions and BSL- 2. Refer to the following site: http://viceprovost.tufts.edu/ibc/exposure-response-plans/acanthamoeba/