Interpretation of X-ray reflectivity spectrum?

Dear community, I performed XRR measurements on thin film AuAgSi alloys deposited on Si substrate, please see attached image. For simulation i included 2 nm of SiO2 on top of the substrate and i...

03 February 2021 5,325 2 View

What is the best GIS tool to calculate connectivity score for a point along aquatic network?

Hello, I am looking for a GIS tool (ArcGIS, QGIS, R, etc.) that will calculate a measure of habitat connectivity for nodes along an aquatic (estuarine) network? My dataset is comprised of a...

12 January 2021 6,107 2 View

Can I sequence PCR products from green masterm mix without purification?

I wonder if anybody has an experience of sequencing PCR products where master mix contains green dye for gel loading. What if I will purify them with enzymes instead of gel extraction or...

18 May 2020 5,831 5 View

How to calculate circular dichroism in VASP?

Is there an option to calculate circular dichroism in VASP. If not in VASP then is some free open source software. I need to calculate the spectra for small inorganic nanoclusters (100-200 atoms)

07 May 2019 9,377 3 View

Does anyone detect the 'salt effect' when determining ammona in seawater, highly saline water or brine?

Does anyone detect the 'salt effect' when determining ammona in seawater, highly saline water or brine? How does the time of colouration change when using the indophenol blue reaction in salt...

09 January 2019 1,932 5 View

There is a probability distribution of a discrete random variable "x", what is the probability that the distribution stay constant during time "t"?

There is a probability distribution of a discrete random variable "x" (is given below), which the probability that the distribution stay constant (with a tolerance of 5%) with the passage of the...

14 October 2016 1,005 3 View

What types of mouse BM cells can adhere?

07 July 2016 9,616 3 View

Why ROC Curves developed by MATLAB tools are not completely consistent with ROC curves plot from "Principles of underwater sound" (Urick, 1983)?

07 July 2016 474 2 View

I'm involved in CVD graphene preparation and I need a gas diagram (Ar, H2 and CH4) for a CVD equipment. Can anyone help me, please?

Actually I have almost everything for the experiment - chemicals, foil, CVD equipment and so on. And here is the thing - how to adjust the flow of gases?Here you can see a part of a receipt from...

16 March 2016 7,373 3 View

Why are my macrophages positive for both CCR7 and CD206?

I have stained mouse BM with F4/80, CD11b, MHCII, CD206 and CCR7. Cells were Fc-blocked. When gated on F4/80_hi/CD11b_med-hi, I see a population that is CCR7+/CD206+ double positive (see...

31 January 2016 1,192 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View