Does anyone knows how many NK cells per mice spleen we can get using the NK isolationKit II from Miltenyi Biotech?
Hi,
you can expect to get around 2Mio NK cells in B6 mice, generally I'd check on the mouse genome database how many NK cells you can expect to get from spleens of different mouse strains.
Did you ask Miltenyi? I'd be interested in what they say?
Best of luck!
Join recently
I'm making my research on metagenomics and I want to find the source of the command 'vsearch merge-pairs' that used to be 'vsearch join-pairs'' How can I cite that?
28 June 2024 3,960 1 View
Hey! I'm looking for ways to compare two kinetic curves over time. My data is composed of neutralizing antibody results during 11 points (vaccine kinetics) in each group (control and...
29 February 2024 9,979 3 View
Hello, We designed a series of tests that aim to assess knowledge in first-year university students, and went through the process of validating each one of them (construct validity, through EFA,...
01 January 2024 4,251 4 View
Hello everyone, I'm currently working with chromatograms on a Shimadzu system and am seeking assistance in converting files saved in either .CDF or .qgd formats to .d format for use in Mass...
12 December 2023 5,663 2 View
I am currently in the process of doing a Western Blot with mESCs after an AHA Assay. I added 25uL of 2xLaemmli buffer and 25uL of AHA buffer (50mM Tris HCL) to my pellet. I usually denature my...
05 December 2023 4,334 3 View
Hi everyone! I am new in lab and I have been having problems with Western Blot, I use a Chemidoc and when I reveal I see nothing, after reincubate, or incubating with a new antibody, the signal is...
29 August 2023 9,130 3 View
Hello everyone. We are using thrombin from bovine plasma (T4648-1KU, sigma). ¿How can we make a stock of 100 U/ml thrombin (0.1% BSA solution)? ¿Should we weigh the powder and reconstitute it...
05 July 2023 2,691 2 View
I use 1x ttbs, wash it 4x for 5 mins and 3x for five mins after transfer, and before blocking. I use everyblot from biorad as the blocking agent and for antibody incubation. Thanks!
04 July 2023 9,343 3 View
I accept suggestions for articles about linalyl acetate films or membranes.
21 December 2022 2,163 0 View
Hi! I am using DADA2 and phyloseq in R and I get slightly different results if I run the commands more than once with the same sample. I am working with 16S rRNA gene amplicons (Illumina, single...
29 November 2022 4,079 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View