Which assays can be perform while doing T cell profiling?

More Rashmi Virkar's questions See All

Is it possible that insecticides from same group and same mode of action may not show cross resistance?

I could not find any cross resistance between two insecticides from OP group, is this possible?

14 January 2021 9,625 3 View

What is the role of top counter electrode when using perovskite thin-films in photoelectrochemical application?

I am currently working on perovskite based photoelectrodes, but I do not want to replace the counter electrodes including Ag, Au, will it have serious issue in the PEC performance?

13 January 2021 3,408 2 View

Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

I was genotyping some mice tail snips. It is a Cre Lox reporter mouse. The Cre is expressed using CRH promoter in the mouse brain and eGFP is the reporter gene. I isolated g-DNA and used it for...

12 January 2021 6,875 3 View

Insecticide resistance without selection pressure, if possible?

What could be possible reasons for insecticide resistance among insects from areas which have very little exposure of insecticides or there is no selection pressure and the areas are rich in...

07 September 2020 1,346 15 View

When to add H2DCFDA dye before or after treatment and why?

When to add ROS generation binding dye H2DCFDA, before or after treatment and why?

07 September 2020 4,962 3 View

Does anyone know a good IHC protocol for FGFR1 detection on floating brain sections?

I am trying to standardize IHC for cFOS and FGFR1 detection on rat brain sections. I use 40 micron sections and have had good results for cFOS detection. But I have not had any positive results...

25 March 2020 582 5 View

Any researcher working on Alkaloids in India?

Any researcher working on the alkaloids(Trichodesmine, Junceine, Riddelline, Senecionine, Seneciphylline, Lycopsamine and Intermidine) in india. Need help regarding this.

03 March 2020 1,060 4 View

How is child protection mechanism offered by the Government of India?

There are many children in India who are in difficult circumstances. The categories included are: homeless children, migrant children, orphaned children, child workers, bonded children, street...

24 January 2020 1,028 1 View

What can be used as a positive control in western blot for beta tubulin?

I am studying the difference in the tubulin in the mutants and want to load a positive control for my blot. looking for suggestions other than GADPH.

09 December 2019 4,249 2 View

The standard quercitin on TLC plate is showing two spots one at the point of origin and one on top.Why is standard showing two spots?

the same standard run in methanol showing single spot.

08 August 2019 3,610 7 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View