I need a protocol for the isolation of leukocytes from intestinal mucosal tissue and which solvent to be used to store the tissues before isolation?

More N. Nivedita's questions See All

Why Negative peak eluted in HPLC analysis?

I'facing problem while doing HPLC analysis due to negative peak was eluted which was corresponding to the principle peak RT. I tried to remove, with different solvents, but no use. i want to know...

26 November 2020 1,960 5 View

Can anyone please tell how to define link for Tuned mass Dampers using Sap2000/Etabs?

Dear Research scholars, I was doing my MTech thesis on pendulum based TMD and I was having trouble defining the link properties. I even followed the tutorials put on CSI website. still, I wasn't...

11 November 2020 8,309 11 View

How to solve "cz freeze python error in main script" Acp in Ansys probelm?

Greetings, I'm currently using the ANSYS 2020R1 crack version. I used this software for at least one month smoothly. But in the last few days, I'm facing this problem. I'm tired to solve this...

09 November 2020 6,431 2 View

What is the suitable optical filter for filtering 120MHz spacing combs from mode-locked laser for f-2f interferometry?

how to do f-2f interferometry from an octave spanning supercontinnum with frequency spacing 120MHz. Which would be the most suitable optical filter in this range for filtering f and 2f components...

12 October 2020 4,470 1 View

How we can design hybrid system between deep learning and swarm intelligence system or deep learning with fuzz?

Hybride intelligent system is good techniques used for solving diffrent optomization problems. Like classification, recognition ....etc Deep learning can be used with and hybrid with other machine...

08 October 2020 4,943 3 View

Why not there is common reference citation format for all journals?

03 October 2020 8,137 3 View

Which force field should be used to simulate protein-protein complex in gromacs?

Hi, I have done a protein-protein docking. Now I want to perform MD in gromacs. I am confused with forcefields. When I tried to findout, everywhere it is written for protein-ligand but I couldn't...

29 September 2020 1,410 3 View

Anyone knows a reliable service who can run my data for me?

Please, if you know such a person or service, kindly email me the contact. I am looking for a reliable service to help me with my data set analyses. Thank you, Y. Essounga: [email protected]

23 September 2020 2,080 3 View

How authentic is this conference, https://www.scientificresearchers.org/icbe-8?fbclid=IwAR2_kI2DGxq7Hlc-Be6gIZKrwgXvjqg2Q5FT2SmIo2yiQecWAcMDpl7GZS0?

Good day, I just to want whether the above conference is a fake or not Regards

17 September 2020 3,912 7 View

Is it possible to run a doped system in CASTEP without creating supercell structure?

I was run a doped system in CASTEP without supercell and it was successfully optimized. But, I am not fully understood when I have to create a supercell. The unit cell I had used contain 28...

06 July 2020 7,657 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Hannah Wessel

Hi there, I have never before stored the tissue for any length of time before isolation of leukocytes - what were you hoping to use them for?

Here is a good protocol that we use in the lab routinely for isolating mucosal myeloid cells for flow cytometry analysis and it also works nicely for T cells and B cells - you don't need to do any enrichment after isolation.

http://www.ncbi.nlm.nih.gov/pubmed/27142011

Hope that helps!

Grant Steven Jones

Here is an additional protocol you may find of interest that describes fractionation of immune cells (focused on mononuclear phagocytes) from different layers of the intestine. I have experienced much better yields of lamina propria cells after switching to this protocol.

https://www.ncbi.nlm.nih.gov/pubmed/27479657

N. Nivedita

Hannah Wessel and Grant Steven Jones, I am hoping to use them only for metabolite analysis, should I be following the same protocol or are there any other less tedious protocols?