I am trying to prepare an anisomycin solution for injection into the brains of my rats. I am dissolving the ANI in equimolar HCl ( 1 mmol of ANI for 1mmol of HCl). The ANI dissolves easily in HCl. I then diluted the HCl a bit with saline, which was also no problem, but when I adjusted the pH of my solution with NaOH the ANI fell out of solution again.
Any suggestion?
Thanks