Hello. I need an advise. Is there any method for the detection of CA 15-3 and PSA except immunoassay? Thanks
Is there a reason you cannot use an immunoassay?
Are you saying you want an assay for PSA and CA 15-3 that does not involve using antibodies?
The other method is PCR, RealTime or RT-PCR. But, first, you should be aware that immunoassays are being used for diagnostic because it is more sensitive method, and this matter for blood tests. PSA level is measured quantitatively via ELISA in blood serum for prostate cancer non-invasive early diagnostic. And second, you will need to make primers design for PCR as Muc1 (CA 15-3) has many variants to detect, and PSA is normally detected via immunoassays, so ready PCR systems do not exist, moreover for these two antigenes in one test.
It is also possible to detect both PSA and CA 15-3 in standard nucleotide sequencing, 2D-SDS-PAGE and MALDI proteomics.
Thanks, Tim, there's no reason I can't use immunoassay. I just wanted to learn all the possibilities before to start a new research project.
Elena, thanks for your help. It was very informative and useful. I'll be working on this question more deeper. And no, I don't need to detect these two antigens in one test
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