I have gold coated quartz crystals that I want to bind with antibodies to perform an immunoassay. The crystals are quite expensive and I would like to be able to clean the surfaces and reuse them.
You can try stripping buffer. After seeing the composition, you would be able to understand the property of buffer.
Jarosite is obtained from bioleaching residue and I want to determine if there are increased active sites biogenic jarosite compared to chemical synthetic jarosite.
10 July 2024 8,132 2 View
Scales?
10 July 2024 1,176 2 View
Hi, I would like to gain an insight on the various methods of measuring biomass and growth of Aspergillus oryzae, which you have personally tried and would gladly recommend. Thanks.
08 July 2024 6,057 2 View
Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...
27 June 2024 5,068 1 View
The major causes of post harvest loss of produce in Ghana.
20 June 2024 6,445 5 View
Hi, I want to analyze the protein expression levels of HPV16 E6/E7 in the cervical cancer cell lines SiHa and CaSki after treatment with silencing RNA for this oncogene. Can anyone recommend a...
03 June 2024 9,579 3 View
I'm implanting Alzet pumps in mice with a brain infusion cannula to deliver into the ICV space. I am familiar with the technique that includes removing the pump from the tubing and then using a...
22 May 2024 7,121 3 View
Hi, I am performing optimization calculations in VASP for oxides. After my calculation was finished, I checked the OUTCAR file, and the optimization finished well. But when I checked the other...
29 April 2024 1,506 2 View
Hello, I have data in SPSS from a scale I did, I have 1 variable which is age (separated into 3 categories using values of 1-3), a 2nd variable which is gender (again separated into 3 categories...
19 April 2024 9,983 3 View
We are trying to use the Life Diagnostics Trout HSP70-23 to look at Heat Shock Protein 70 in fish red blood cells to look at physiological changes in response to stressors. I have found very...
08 April 2024 1,134 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Please note it
29 July 2024 4,920 3 View
https://www.cleanteqwater.com/resources/case-studies/gold-mine-australia/
29 July 2024 5,161 1 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View