Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Has annyone try to reuse EveryBlot Blocking Buffer (Bio-rad) for fluorescence western blotting?

Hello to every one, Our lab has recently switched our western blot technique from chemiluminescence to fluorescence. Therefore, we have to optimize the blocking buffer. We have successfully try...

05 February 2021 7,886 1 View

How to use Image Lab to take an image for the bands and the ladder in the same image merged together in Western Blot?

Hello everyone. I was trying to use Image Lab software to take an image of both the ladder and the samples merged together in one image to see the position of the bands according to the ladder MW,...

04 February 2021 9,996 2 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Colorimteric and enzymatic method ?

First of all, thank you for your consideration about this question. I've been working on development of in-vitro diagnostic medical device as research engineer since 2013. Especially, my major is...

14 January 2021 3,885 4 View

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

06 January 2021 5,769 3 View

IHC-P staining of the viral infection in the mouse pancreas?

Hi, I'm trying to stain a viral infection of the pancreas in paraffin sections of adult (3 month old) BALB/c mice. Prior to starting my histology analysis, I have titrated the organ to confirm...

10 December 2020 2,198 3 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View