Hi to everyone!
Does anybody know optimal buffer for detecting HIV-IgG with lateral flow immunoassay using saliva as a specimen type?
I think that i need a buffer for LFIA becouse saliva too viscous.
thank you!
If in doubt, simply use PBS. That should keep all proteins happy. If background is high, add 0.1% triton or tween. If you need a preservative, 0.1% sodium azide should do (mersalyl if you detect by peroxydase).
Valeriy,
why not examining the dependence of the primary antibody (or its conjugate you use) activity on pH? Then make a choice for a buffer.
Good luck!
P.S. Let say, pH 5 (acetate buffer), pH 7 (phosphate), and pH 9 (borate).
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