When I defrost my plasma samples from -80, I do so on ice, slowly. Then I centrifuge the samples. Upon centrifugation, the plasma samples will have a white sticky substance layer at the top, but sometimes throughout. I avoid this with my tip as best as possible.
My results were anything but consistent. I am wondering should I vortex to force the sticky substance into solution? Does this substance, likely precipitate contain the cytokines of interest and that could explain my results?
Some of my plasma samples are over 10 years old, but have never been thawed before. SO I get how the inconsistencies could be the age of the samples.
Hey Camilla,
we use plasma samples from mice for cytokine measurements (ELISA or Procarta Multiplex) regulary. We store our samples at -20°C for short term storage and at - 80°C for long term storage.
Independently, we thaw them at room temperature and always vortex the sample before transfering it into the wells (for ELISA or Multiplex) and we never encountered problems. I think rigorous thawing and vortexing is the key to your problem.
All the best and stay healthy,
Marc
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