While using EDTA decalcify bone samples from Mus musculus, how much volume of EDTA is it recomended per piece volume? For the fixation with formaldehyde the normal is arround 20x...
I used to decalcify shep tibia. The percentage od EDTA is 14% when you make it. Use the pH and heat to make the solution otherwise takes forever. Then correct pH to neutral. Put the bone in a sonicator with the edta and let it go overnight or 8h and the calcium will disappear. If you de calcify bone for microscopy instead a few drops on the slide and leave it for 5min and will be de-calcified