We are processing pig lungs for ICC. We inflate the lungs with air, then cut a piece and fix it in paraformaldehyde. The next day, we change it to 30% sucrose and wait until the tissue sinks to embed it in OCT. However, most of the lung pieces do not sink after 3-5 days. Does someone have had the same problem with lung tissue? Should we be using a different sucrose concentration or a different cryoprotectant?
Thank you so much!