Can anyone suggest me a pre-sorting and post-sorting buffer for H9C2 cardiomyocytes cell lines? I'm using BD Facs Melody
Thanx
Hi, I want to detect Granzyme B after CD8+ stimulation by Dynabeads. My question is : Ho long should I incubate my Cd8+ with beads to detect (flow cytometry) GzB? and when should I incubate cells...
03 February 2022 1,844 2 View
Hi, Just a little question about ROS. I measured ROs in untreated and treated (2T) with Ultrasound, I've observed 2 peaks in DCFH-DA in WT. It's normal? In some experiments, the second peak...
21 December 2021 6,483 1 View
Hi, Just a little question about ROS. I measured ROs in untreated and treated (2T) with Ultrasound, I've observed 2 peaks in DCF-DA in WT. It's normal? In some experiments, the second peak...
18 December 2021 4,074 1 View
Why I still having negative gfp mioblast sorted after gating on positive gfp over 10^4 signal? Fig1 negative population fig 2 pre sorting fig 3 post sorting. Facs melody nozzle 100 um no aggregates..
15 November 2021 5,035 3 View
Does anyone have experience with MCL-1 detection in resting lymphocytes by flow cytometry? I've tried to stimulate peripheral lymphocytes with Il-7 10 ng/ml for 6 hours and with CD3/CD28 beads for...
01 January 1970 5,124 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View